{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:51:29Z","timestamp":1790682689731,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,5,5]],"date-time":"2017-05-05T00:00:00Z","timestamp":1493942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1101046"],"award-info":[{"award-number":["1101046"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The El Plomo mummy was a pre\u2010Columbian Incan child who was found mummified in the Andes Mountains above an altitude of 17,700 feet. In the environment, natural mummification occurred due to low temperatures and strong winds. Dating measurements (relative dating) by experts from the National Museum of Natural History of Chile established that the mummified body corresponds the Inca period (1,450 to 1,500 AD). In 2003, the body was transferred to the University of Chile Medical School for exhaustive medical examination. Tissue samples from the right quadriceps muscle were extracted and fixed in glutaraldehyde and postfixed in osmium tetroxide to obtain ultrathin sections to be observed by transmission electron microscope. Images were recorded on photographic paper, digitalized and analyzed by experts on morphology. Results showed a preservation of cell boundaries in striated muscle cells, but specific subcellular organelles or contractile sarcomeric units (actin and myosin) were unable to be recognized. However, the classical ultrastructural morphology of the polypeptide collagen type I was preserved intact both in primary and secondary organization. Therefore, we concluded that the process of natural mummification by freezing and strong winds is capable of damaging the ultrastructure of muscle cells and preserving collagen type I intact.<\/jats:p>","DOI":"10.1002\/jemt.22879","type":"journal-article","created":{"date-parts":[[2017,5,5]],"date-time":"2017-05-05T11:26:50Z","timestamp":1493983610000},"page":"898-903","source":"Crossref","is-referenced-by-count":4,"title":["Ultrastructural preservation of tissues and their reaction to the infection with trichinella in the El Plomo mummy: Muscle fiber ultrastructure and trichinosis\/mummy of the Cerro El Plomo"],"prefix":"10.1002","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7822-2867","authenticated-orcid":false,"given":"Rodr\u00edguez","family":"H\u00e9ctor","sequence":"first","affiliation":[{"name":"Facultad de Medicina Universidad de Chile. Chile Independencia 1027 Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Espinoza\u2010Navarro","family":"Omar","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Universidad de Tarapac\u00e1 General Vel\u00e1zquez 1775 Arica Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Instituto de Estudios Cient\u00edficos de Momias de Madrid. Espa\u00f1a Urbanizaci\u00f3n Las Rosas N\u00b0 37 Madrid Espa\u00f1a"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Castro","family":"Mario","sequence":"additional","affiliation":[{"name":"DIBAM. Chile Recoleta Dom\u00ednica 683 Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,5]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Australian Museum. (2009). Retrieved fromhttp:\/\/australianmuseum.net.au\/The-origins-of-mummification-in-ancient-Egypt. September 22 2009."},{"key":"e_1_2_7_3_1","unstructured":"Cabezas A.(1986). El Santuario de Altura Inca Cerro El Plomo. Thesis for the degree of Licensee in Archaeology and Chilean Prehistory Universidad de Chile. Santiago Chile. pp. 160."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2006.00607.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8644(199808)106:4<521::AID-AJPA7>3.0.CO;2-L"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2010.0377"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.20728"},{"key":"e_1_2_7_8_1","unstructured":"Mahajan R. &Amin R.(2011). Mummification: The mummies of ancient Egypt. (http:\/\/library.thinkquest.org\/C0116982\/HTML%20page%20folder\/hmummification.htm). Accesed on April 15 2012."},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2011.01442.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502718103"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01330.x"},{"key":"e_1_2_7_12_1","first-page":"31","article-title":"Protein\u2010energy malnutrition effect and hypobaric stress on ultrastructure of the heart muscle in rats","volume":"49","author":"Ram\u00edrez L. M.","year":"1998","journal-title":"Acta Cientifica Venezolana"},{"key":"e_1_2_7_13_1","first-page":"65","article-title":"Mitochondrial metabolism and ultraestructure of mice's skeletal muscle with caloric proteic restriction","volume":"33","author":"Ramirez L. 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