{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:55:25Z","timestamp":1790193325989,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,5,22]],"date-time":"2017-05-22T00:00:00Z","timestamp":1495411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2003\/04253\u20109"],"award-info":[{"award-number":["#2003\/04253\u20109"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to investigate the effect of cyclosporine A (CsA) therapy on bone healing around osseointegrated implants in the rabbit by means of descriptive histologic and histomorphometric analyses. Eighteen rabbits received one implant each in the right tibia proximal metaphysis. After the bone\u2010healing period of 3 months, 6 randomly selected animals were sacrificed (Group CTL\u201012) before the immunosuppression therapy initiation to obtain a standard of implant integration. Another six randomly selected animals were submitted to a daily subcutaneous (<jats:italic>sc<\/jats:italic>) injection of 10 mg\/kg CsA (Group CsA) whereas the six remaining animals received daily <jats:italic>sc<\/jats:italic> injections of saline solution (Group CTL\u201024). Groups CTL\u201024 and CsA were sacrificed after another 12 weeks. Bone\u2010to\u2010implant contact (BIC), and bone area fraction occupancy (BAFO) within the implant threads were measured. Bone density (BD) and descriptive histological analyses were also accomplished. Rabbits under CsA therapy presented statistically lower percentage of BIC (33.28\u2009\u00b1\u20096.19) compared to the animals of CLT\u201024 (55.6\u2009\u00b1\u200917.73). No differences were found between CsA and CTL\u201012 (38.7\u2009\u00b1\u20097.87). Significant decreased percentage of BAFO between the implant threads in the CsA (48.43\u2009\u00b1\u20099.48) group compared to the CTL\u201024 (72.26\u2009\u00b1\u200911.72) was observed. No differences were found between CsA and CTL\u201012 (61.99\u2009\u00b1\u200913.94) groups. BD analysis showed significant lower BD in the CsA (48.56\u2009\u00b1\u20099.32) group compared to the CTL\u201012 (68.74\u2009\u00b1\u200910.89) and CTL\u201024 (77.96\u2009\u00b1\u20094.96) groups. Collectively, our findings demonstrated that CsA therapy negatively affect the bone healing around osseointegrated implants due to the significant lower values for BIC, BAFO, and BD.<\/jats:p>","DOI":"10.1002\/jemt.22894","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T20:01:21Z","timestamp":1495569681000},"page":"1000-1008","source":"Crossref","is-referenced-by-count":19,"title":["Effect of the long\u2010term administration of Cyclosporine A on bone healing around osseointegrated titanium implants: A histomorphometric study in the rabbit tibia"],"prefix":"10.1002","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1110-6233","authenticated-orcid":false,"given":"Rafael Scaf","family":"de Molon","sequence":"first","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara Sao Paulo State University \u2010 UNESP Araraquara SP Brazil"},{"name":"Department of Rheumatology Rheumatology Research and Advanced Therapeutics, Radboud University Nijmegen Medical Centre 6500 HB Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso Eduardo","family":"Sakakura","sequence":"additional","affiliation":[{"name":"Department of Periodontology Barretos Dental School, Education Foundation of Barretos \u2010 FEB Barretos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Silveira","family":"Faeda","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Implantology Post Graduation Course, University Center of Araraquara \u2010 UNIARA Dental School  SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Sartori","sequence":"additional","affiliation":[{"name":"Department of Dentistry Ponta Grossa State University Ponta Grossa Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Palhares","sequence":"additional","affiliation":[{"name":"Department of Periodontology Barretos Dental School, Education Foundation of Barretos \u2010 FEB Barretos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rogerio","family":"Margonar","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Implantology Post Graduation Course, University Center of Araraquara \u2010 UNIARA Dental School  SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Elcio","family":"Marcantonio","sequence":"additional","affiliation":[{"name":"Department of Diagnosis and Surgery, School of Dentistry at Araraquara Sao Paulo State University \u2010 UNESP Araraquara SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,22]]},"reference":[{"issue":"1","key":"e_1_2_7_2_1","first-page":"e13","article-title":"Parameters for successful implant integration revisited part II: Algorithm for immediate loading diagnostic factors","volume":"12","author":"Bahat O.","year":"2010","journal-title":"Clinical Implant Dentistry and Related Research"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-006-0049-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/357695a0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2008.01695.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2012.02467.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2016.46.3.176"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.11.1618"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.10.1391"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-74912003000400012"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa067411"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1563\/AAID-JOI-D-09-00098"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2006.00175.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-015-9970-z"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199345060-00007"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070466"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1994.tb00312.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.2.189"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1258\/002367786780865692"},{"issue":"2","key":"e_1_2_7_20_1","first-page":"69","article-title":"Integration of screw implants in the rabbit: A 1\u2010year follow\u2010up of removal torque of titanium implants","volume":"2","author":"Johansson C.","year":"1987","journal-title":"The International Journal of Oral & Maxillofacial Implants"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198912213212507"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2012.02.010"},{"issue":"2","key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/S0022-3565(25)11038-0","article-title":"Cyclosporine A inhibits bone resorption in cultured neonatal mouse calvaria","volume":"243","author":"Klaushofer K.","year":"1987","journal-title":"The Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00334490"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-123-5-2571"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650040314"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008505-200204000-00020"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-128-3-1638"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-016-1936-7"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0192-0561(98)00004-6"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(98)00081-3"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(94)90053-1"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2006.01253.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.7.976"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050290"},{"issue":"3","key":"e_1_2_7_36_1","first-page":"71","article-title":"Cyclosporine\u2010a and bone density around titanium implants: A histometric study in rabbits","volume":"2","author":"Sakakura C. E.","year":"2011","journal-title":"Journal of Osseointegration"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-124-5-2179"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.5.659"},{"issue":"6","key":"e_1_2_7_39_1","first-page":"281","article-title":"Immunosuppressive drugs after solid organ transplantation","volume":"71","author":"Sandwijk M. S.","year":"2013","journal-title":"The Netherlands Journal of Medicine"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.2.281"},{"issue":"4","key":"e_1_2_7_41_1","first-page":"CD003961","article-title":"Tacrolimus versus cyclosporin as primary immunosuppression for kidney transplant recipients","author":"Webster A.","year":"2005","journal-title":"Cochrane Database of Systematic Reviews"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjemt.22894","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.22894","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:51:22Z","timestamp":1760633482000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.22894"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,22]]},"references-count":40,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1002\/jemt.22894"],"URL":"https:\/\/doi.org\/10.1002\/jemt.22894","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,22]]}}}