{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:33:28Z","timestamp":1784043208635,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,5,26]],"date-time":"2017-05-26T00:00:00Z","timestamp":1495756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/11595\u20102"],"award-info":[{"award-number":["2012\/11595\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/18203\u20102"],"award-info":[{"award-number":["2012\/18203\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim was to evaluate the cytocompatibility of the AH Plus and Sealapex associated with amoxicillin (AA) in a quantity of 10% by total weight of the sealers, the flow and setting time of these sealers with 10%\u20100.25% AA and antibiofilm activity of these sealers associated with AA in a concentration that does not alter their physical properties (1%). Cytocompatibility was assessed by MTT, neutral red and cytoskeletal fluorescence assays. Setting time and flow was evaluated using the specifications of ISO 6876\/2012. For the antibiofilm evaluation, materials were placed in direct contact with <jats:italic>E. faecalis<\/jats:italic> biofilm induced on dentin blocks. The incorporation of AA into the sealers did not diminish the viability of the fibroblasts and did not cause structural changes in the cytoskeletal. The flow of AH Plus\u2009+\u2009AA at 1.0; 0.5 and 0.25%, and of Sealapex\u2009+\u2009AA at 5.5; 2.5; 1.0; 0.5 and 0.25% were within the specifications of the ISO 6876. AH Plus, and AH Plus\u2009+\u2009AA at 1.0; 0.50 and 0.25% presented a setting time of 730, 439, 455, and 474 min., respectively. Sealapex \u2010 pure or associated with AA did not set completely. The addition of 1% AA to the sealers did not increase their antibiofilm activity. It was concluded that the incorporation of 10% AA caused no toxic effects on fibroblasts, but harmed the physical properties. The addition of AA in concentration that does not affect the physical properties of the sealers did not increase their antibiofilm activity.<\/jats:p>","DOI":"10.1002\/jemt.22898","type":"journal-article","created":{"date-parts":[[2017,5,26]],"date-time":"2017-05-26T08:36:44Z","timestamp":1495787804000},"page":"1036-1048","source":"Crossref","is-referenced-by-count":12,"title":["Cytocompatibility, physical properties, and antibiofilm activity of endodontic sealers with amoxicillin"],"prefix":"10.1002","volume":"80","author":[{"given":"Carolina","family":"Andolfatto","sequence":"first","affiliation":[{"name":"Araraquara School of Dentistry, Department of Restorative Dentistry UNESP \u2013 S\u00e3o Paulo State University Araraquara SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idomeo","family":"Bonetti\u2010Filho","sequence":"additional","affiliation":[{"name":"Araraquara School of Dentistry, Department of Restorative Dentistry UNESP \u2013 S\u00e3o Paulo State University Araraquara SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iracilda Zeppone","family":"Carlos","sequence":"additional","affiliation":[{"name":"Araraquara School of Pharmaceutical Sciences, Department of Clinical Analysis UNESP \u2013 S\u00e3o Paulo State University Araraquara SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane Maria","family":"Guerreiro\u2010Tanomaru","sequence":"additional","affiliation":[{"name":"Araraquara School of Dentistry, Department of Restorative Dentistry UNESP \u2013 S\u00e3o Paulo State University Araraquara SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milton Carlos","family":"Kuga","sequence":"additional","affiliation":[{"name":"Araraquara School of Dentistry, Department of Restorative Dentistry UNESP \u2013 S\u00e3o Paulo State University Araraquara SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Borges Cunha","family":"Tormin","sequence":"additional","affiliation":[{"name":"Araraquara School of Dentistry, Department of Restorative Dentistry UNESP \u2013 S\u00e3o Paulo State University Araraquara SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7030-3718","authenticated-orcid":false,"given":"Gisele","family":"Faria","sequence":"additional","affiliation":[{"name":"Araraquara School of Dentistry, Department of Restorative Dentistry UNESP \u2013 S\u00e3o Paulo State University Araraquara SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,26]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"ASTM(2008). 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