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In this article, we describe a simplified method of preparation of small intestinal mucosa tissues for observations in a scanning electron microscope. We emphasized the most important points in the preparation process that, when ignored, may result in formation of numerous artifacts and the inability to analyze the samples reliably. The developed technique facilitates proper animal tissue sampling in the field conditions, reducing the time of tissue collection and sample preparation as well as the total process costs. The fixative of choice, that is, buffered formalin, fixes, and stiffens the processed tissues properly, which is especially important in preservation of long, highly hydrated intestinal villi without shrinkage artifacts. The method described has been successfully used in comparative studies of the development of small intestines in mammals (pigs, mice, rats), reptiles, and birds (hens).<\/jats:p>","DOI":"10.1002\/jemt.23141","type":"journal-article","created":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T07:22:19Z","timestamp":1538983339000},"page":"1325-1331","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A simplified method of preparation of mammalian intestine samples for scanning electron microscopy"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1836-3734","authenticated-orcid":false,"given":"Tomasz H.","family":"Skrzypek","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Environmental Sciences, Laboratory of Confocal and Electron Microscopy, Center for Interdisciplinary Research John Paul II Catholic University of Lublin  Lublin Poland"},{"name":"Department of Biotechnology and Environmental Sciences, Laboratory Ultrastructure and Electron Microscopy John Paul II Catholic University of Lublin  Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waldemar","family":"Kazimierczak","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Environmental Sciences, Laboratory of Biocontrol, Application and Production of EPN, Center for Interdisciplinary Research John Paul II Catholic University of Lublin  Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,8]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"A study of fixation and dyeing","author":"Baker J. 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