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In this study, HPF for anesthetized living <jats:italic>Drosophila<\/jats:italic> was performed by placing them directly on the carrier of the HPF unit and exposing them to light. Frozen <jats:italic>Drosophila<\/jats:italic> were freeze substituted, and their compound eyes were examined by transmission electron microscopy. The ultrastructures of ommatidia composed of photoreceptor cells were well preserved. The location of the cytoplasmic organelles inside the photoreceptor cells was observed. In some photoreceptor cells in ommatidia of the light\u2010exposed <jats:italic>Drosphila<\/jats:italic>, the cytoplasmic small granules were localized nearer the base of rhabdomeres, compared with those of the nonlight\u2010exposed <jats:italic>Drosophila<\/jats:italic>. Thus, HPF with the direct insertion of living <jats:italic>Drosophila<\/jats:italic> under light exposure into the HPF machine enabled us to examine changes to functional structures of photoreceptor cells that occur within seconds.<\/jats:p>","DOI":"10.1002\/jemt.23166","type":"journal-article","created":{"date-parts":[[2018,12,24]],"date-time":"2018-12-24T01:00:37Z","timestamp":1545613237000},"page":"244-249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Electron microscopic observation of photoreceptor cells in directly inserted anesthetized <i>Drosophila<\/i> into a high\u2010pressure freezing unit"],"prefix":"10.1002","volume":"82","author":[{"given":"Hitomi","family":"Terada","sequence":"first","affiliation":[{"name":"Health Science Division, Department of Medical Sciences Shinshu University Graduate School of Medicine, Science and Technology  Matsumoto City Nagano Japan"},{"name":"Division of Sciences 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