{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:54:19Z","timestamp":1787064859768,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,12,29]],"date-time":"2018-12-29T00:00:00Z","timestamp":1546041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/25133\u20101"],"award-info":[{"award-number":["2016\/25133\u20101"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    and\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    have been associated with cases of secondary and persistent root canal infections, been resistant to calcium hydroxide. So, the evaluation of the susceptibility of these microorganisms biofilms to new drugs is an important practice for establishing the best drug and consequently success of treatment. For this, in vitro biofilm formation of\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    and\n                    <jats:italic>C. albicans<\/jats:italic>\n                    was induced separately on blocks obtained from bovine teeth. After the period of specimen incubation for biofilm maturation, the samples were immersed in the pastes: 1 \u2013 calcium hydroxide (CH), 2 \u2013 chlorhexidine (C), 3 \u2013 ciprofloxacin (CP), 4 \u2013 metronidazole (MT), 5 \u2013 ketoconazole (KE), 6 \u2013 double antibiotic (DB), 7 \u2013 triple antibiotic (TA), 8 \u2013 ciprofloxacin\u2009+\u2009ketoconazole (CPKE); 9 \u2013 ciprofloxacin\u2009+\u2009metronidazole\u2009+\u2009ketoconazole (CPMTKE), 10 \u2013 metronidazole\u2009+\u2009ketoconazole (MTKE), and 11 \u2013 control (CO) for 7 days. Next, the specimens were live\/dead stained for analysis by confocal microscopy. By means of the Bioimage program, the biovolume and percentage of live cells were measured. The data were statistically compared (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u2009.05). For the\n                    <jats:italic>C. albicans<\/jats:italic>\n                    biofilm, the best antimicrobial action was found for MTKE, CPKE, and MT groups. Whereas for\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    biofilm, the lowest percentage of live bacteria was found in TA, DB, and CP groups; however, KE, CPKE, CPMTKE, and MTKE groups shown to be effective. The authors concluded calcium hydroxide paste and chlorhexidine was not effective for both biofilms. The MTKE and CPKE pastes presented effectiveness for both biofilms. TA and DB pastes were effective just in the\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    biofilms.\n                  <\/jats:p>","DOI":"10.1002\/jemt.23192","type":"journal-article","created":{"date-parts":[[2018,12,31]],"date-time":"2018-12-31T17:12:15Z","timestamp":1546276335000},"page":"494-500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Antimicrobial activity of intracanal medications against both\n                    <i>Enterococcus faecalis<\/i>\n                    and\n                    <scp>\n                      <i>Candida albicans<\/i>\n                    <\/scp>\n                    biofilm"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5040-5776","authenticated-orcid":false,"given":"Rafaela Fernandes","family":"Zancan","sequence":"first","affiliation":[{"name":"Department of Restorative Dentistry, Dental Materials and Endodontics Bauru Dental School, University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Henrique Souza","family":"Calefi","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Dental Materials and Endodontics Bauru Dental School, University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0049-8657","authenticated-orcid":false,"given":"Mariana Maciel Batista","family":"Borges","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Dental Materials and Endodontics Bauru Dental School, University of S\u00e3o Paulo  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Ribeiro Milanda","family":"Lopes","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Dental Materials and 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