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During stressful periods, painful thresholds of masticatory muscles in individuals suffering muscle facial pain are significantly lower than in controls, but the exact physiologic mechanism underlying this relation remains unclear. Our hypothesis is that chronic unpredictable stress and masticatory hypofunction induce morphologic and metabolic masseter muscle changes in rats. For test this hypothesis, adult Wistar rats were submitted to chronic unpredictable stress and\/or exodontia of left molars and the left masseter muscle was removed for analysis. The parameters evaluated included ultrastructure, oxidative level, metabolism activity and morphological analysis in this muscle. Our data show by histological analysis, that stress and exodontia promoted a variation on diameters and also angled contours in masseter fibers. The masticatory hypofunction increased oxidative metabolism as well as decreased reactive species of oxygen in masseter muscle. The ultrastructural analysis of muscle fibers showed disruption of the sarcoplasmic reticulum cisterns in certain regions of the fiber in stress group, and the disappearance of the sarcoplasmic reticulum membrane in group with association of stress and exodontia. Our findings clarify mechanisms by which chronic stress and masticatory hypofunction might be involved in the pathophysiology of muscular dysfunctions. Masticatory hypofunction influenced oxidative stress and induced oxidative metabolism on masseter muscle, as well as altered its fiber morphology. Chronic stress presented malefic effect on masseter morphology at micro and ultra structurally. When both stimuli were applied, there were atrophic fibers and a complete mitochondrial derangement.<\/jats:p>","DOI":"10.1002\/jemt.23196","type":"journal-article","created":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T09:40:17Z","timestamp":1549878017000},"page":"530-537","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Exodontia\u2010induced muscular hypofunction by itself or associated to chronic stress impairs masseter muscle morphology and its mitochondrial function"],"prefix":"10.1002","volume":"82","author":[{"given":"Yamba Carla Lara","family":"Pereira","sequence":"first","affiliation":[{"name":"Centro Universit\u00e1rio Luterano de Palmas  CEULP, Tocantins Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glauce Crivelaro","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Morphology, Physiology and Basic Pathology of Dentistry School of 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