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In this study, we describe a strategy to adsorb immunoglobulin G (IgG) anti\u2010<jats:italic>Brucella<\/jats:italic> antibodies onto a silicon surface, oriented by protein A obtained from <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content> (SpA). X\u2010ray photoelectron spectroscopy and atomic force microscopy were used to characterize topographically, morphologically, and chemical changes of the sensor functionalization. The activity of the biosensor was assessed by confocal microscopy, scanning electronic microscopy, and bacteria capture assays (BCA). According to the BCA, the efficiency of <jats:italic>Brucella abortus<\/jats:italic> detection with the SpA\u2010IgG anti <jats:italic>Brucella<\/jats:italic> biosensor was three\u2010fold higher than that of the random orientated IgG anti <jats:italic>Brucella<\/jats:italic> biosensor. The limit of detection was 1\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> CFU\/ml. These data show that the orientation of antibodies immobilization is crucial to developing immunosensors for bacterial antigen detection as <jats:italic>Brucella<\/jats:italic> spp and improve its sensibility level. Functionalization with protein A increases <jats:italic>Brucella<\/jats:italic> detection by an antibody\u2010coated surface. Functionalized silicon surface for <jats:italic>Brucella<\/jats:italic> detection was characterized by atomic force microscopy, X\u2010ray photoelectron spectroscopy and confocal microscopy.<\/jats:p>","DOI":"10.1002\/jemt.23206","type":"journal-article","created":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T03:45:35Z","timestamp":1547437535000},"page":"586-595","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Detection of <i>Brucella abortus<\/i> by a platform functionalized with protein A and specific antibodies IgG"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7490-0042","authenticated-orcid":false,"given":"Shantal Lizbeth","family":"Baltierra\u2010Uribe","sequence":"first","affiliation":[{"name":"Departamento de Inmunolog\u00eda. 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