{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:18:32Z","timestamp":1790374712828,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Death of osteocytes is synonymous of bone death. Aseptic osteonecrosis of the femoral head is a lesion characterized by the death of osteocytes occurring after major vascular changes. The evolution may lead to hip osteoarthritis, which requires total hip arthroplasty in most cases. Evolution of aseptic osteonecrosis in four radiological stages is well known. We analyzed 24 femoral heads from patients with osteonecrosis or osteoarthritis, retrieved at the time of surgery for a hip arthroplasty. The aim of the study was to clearly identify the necrotic bone from the living bone in the histological samples. The femoral heads were sawed, and a large sample was harvested in the superior zone; it was stained en\u2010bloc with rhodamine dissolved in formalin to make the osteocytes fluorescent under UV light microscopy. Undecalcified sections, 7 \u03bcm thick, were obtained on a heavy\u2010duty microtome. A micrographic analysis using two UV excitation wavelengths visualized the living osteocytes (in green) and the bone matrix (in blue). A simple method to prepare combined images is described. In addition, the blocks can be analyzed by confocal microscopy to visualize more details. It is possible to identify at low magnification the osteocytes within the bone matrix and the osteonecrotic areas where osteocytes have disappeared. Identification of osteocytes showed that newly formed bone packets are laid on dead trabeculae in patients with aseptic osteonecrosis or with osteoarthritis. In the osteosclerotic areas, the enlarged trabeculae have a dead central core surrounded by recently apposed bone structure units.<\/jats:p>","DOI":"10.1002\/jemt.23379","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T01:06:20Z","timestamp":1569978380000},"page":"2072-2078","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Osteocyte staining with rhodamine in osteonecrosis and osteoarthritis of the femoral head"],"prefix":"10.1002","volume":"82","author":[{"given":"Louis","family":"Rony","sequence":"first","affiliation":[{"name":"Groupe Etudes Remodelage Osseux et bioMat\u00e9riaux, GEROM, EA\u20104658, SFR\u20104208, Univ\u2010Angers, IRIS\u2010IBS Institut de Biologie en Sant\u00e9, CHU\u2010Angers  Angers France"},{"name":"Service Commun d'Imagerie et Analyses Microscopiques, SCIAM, SFR\u20104208, Univ\u2010Angers, IRIS\u2010IBS Institut de Biologie en Sant\u00e9, CHU d'Angers  Angers France"},{"name":"D\u00e9partement de Chirurgie Osseuse CHU\u2010Angers  Angers France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolphe","family":"Perrot","sequence":"additional","affiliation":[{"name":"Service Commun d'Imagerie et Analyses Microscopiques, SCIAM, SFR\u20104208, Univ\u2010Angers, IRIS\u2010IBS Institut de Biologie en Sant\u00e9, CHU d'Angers  Angers France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Hubert","sequence":"additional","affiliation":[{"name":"Groupe Etudes Remodelage Osseux et bioMat\u00e9riaux, GEROM, EA\u20104658, SFR\u20104208, Univ\u2010Angers, IRIS\u2010IBS Institut de Biologie en Sant\u00e9, CHU\u2010Angers  Angers France"},{"name":"D\u00e9partement de Chirurgie Osseuse CHU\u2010Angers  Angers France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5886-9513","authenticated-orcid":false,"given":"Daniel","family":"Chappard","sequence":"additional","affiliation":[{"name":"Groupe Etudes Remodelage Osseux et bioMat\u00e9riaux, GEROM, EA\u20104658, SFR\u20104208, Univ\u2010Angers, IRIS\u2010IBS Institut de Biologie en Sant\u00e9, CHU\u2010Angers  Angers France"},{"name":"Service Commun d'Imagerie et Analyses Microscopiques, SCIAM, SFR\u20104208, Univ\u2010Angers, IRIS\u2010IBS Institut de Biologie en Sant\u00e9, CHU d'Angers  Angers France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10]]},"reference":[{"issue":"1","key":"e_1_2_7_2_1","first-page":"41","article-title":"Anatomo\u2010clinical (radiological and etiological) forms of so\u2010called primary chronic ischemia and osteonecrosis of the upper femoral epiphysis","volume":"38","author":"Arlet J.","year":"1971","journal-title":"Revue du Rhumatisme et des Maladies Ost\u00e9o\u2010Articulaires"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1971.221.3.669"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1179\/his.2000.23.1.45"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-013-9774-y"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2010.09.007"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.320"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2016.09.003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.83B3.0830419"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.47B4.749"},{"key":"e_1_2_7_11_1","first-page":"111","volume-title":"Bone cancer: Progression and therapeutic approaches","author":"Chappard D.","year":"2014"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1179\/his.1991.14.2.117"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2004.05.024"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kem191"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-2838-8_42"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.148"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2818.1999.00457.x"},{"key":"e_1_2_7_18_1","first-page":"219","volume-title":"Conn's biological stains, a handbook of dyes, stains and Fluorochromes for use in biology and medicine","author":"Horobin R. 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