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Silver as one of the nontoxic, safe inorganic antibacterial agents and can serve as replacement of antibiotics. Present research is based on biogenic synthesis of silver nanoparticles (Ag\u2010NPs) as potential antibiotics from fungal metabolites of <jats:italic>Penicillium oxalicum<\/jats:italic>. We used different analytical techniques X\u2010ray diffraction (XRD) and scanning electron microscopy (SEM) for characterization of biosynthesized silver nanoparticles. Furthermore, the antibacterial activity of biosynthesized silver nanoparticles was checked against <jats:italic>Staphylococcus aureus<\/jats:italic>, <jats:italic>S. dysenteriae<\/jats:italic>, and <jats:italic>Salmonella typhi<\/jats:italic> by using well diffusion method and UV visible spectrophotometer. Maximum zone of inhibition recorded against <jats:italic>S. aureus<\/jats:italic>, <jats:italic>Shigella dysenteriae<\/jats:italic> was 17.5\u2009\u00b1\u20090.5\u2009mm (mm) for both species and 18.3\u2009\u00b1\u20090.60\u2009mm for <jats:italic>Salmonella typhi<\/jats:italic>. The biosynthesized silver nanoparticles of <jats:italic>P. oxalicum<\/jats:italic> showed excellent antibacterial activity. It was concluded from our results that biosynthesized silver nanoparticles have significant potential and might be useful for a wide range of biological applications such as bactericidal agent against resistant bacteria, preventing infections, healing wounds, and anti\u2010inflammation.<\/jats:p>","DOI":"10.1002\/jemt.23390","type":"journal-article","created":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T17:26:24Z","timestamp":1571246784000},"page":"72-80","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":196,"title":["Fungal mediated synthesis of silver nanoparticles and evaluation of antibacterial activity"],"prefix":"10.1002","volume":"83","author":[{"given":"Nosheen","family":"Feroze","sequence":"first","affiliation":[{"name":"Department of Biotechnology Mohi\u2010Ud\u2010Din Islamic University  Nerian Sharif 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