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We prepared Cadmium oxide nanoaprticles (CdO NPs) utilizing <jats:italic>Penicillium oxalicum<\/jats:italic>, and cadmium acetate solution via coprecipitate method. The elemental composition and morphology of these synthesized CdO NPs were examined through X\u2010ray diffraction (XRD), UV\u2013Vis absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), and Energy dispersive spectroscopy (EDS). Furthermore, we evaluated the bactericidal potential of prepared CdO NPs using <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E.coli<\/jats:italic>), <jats:italic>Staphylococcus aureus<\/jats:italic> (<jats:italic>S.aureus<\/jats:italic>), <jats:italic>Bacillus cereus<\/jats:italic> (<jats:italic>B.cereus<\/jats:italic>), and <jats:italic>Pseudomonas aeruginosa<\/jats:italic> (<jats:italic>P. aeruginosa<\/jats:italic>). Dimethyl sulfoxide was used as negative control while erythromycin was used as positive control. The XRD spectrum revealed cubic crystalline nanoparticles with 22.94\u2009nm size and UV showed absorbance peak at 297\u2009nm with 2.5\u2009eV band gap energy. FTIR depicted O\u2500H and carboxylic groups along with CdO stretching vibration. EDS showed the presence of organic compounds on Cd and O over NPs surface. SEM results revealed the spherical shape of the CdO NPs. The synthesized NPs exhibited highly potent bactericidal activity against selected strains and demonstrated less optical density of 0.086 after 24\u2009hr. Owing to the significant antibacterial activity of CdO NPs, the broad application prospects of these nanoparticles CdO NPs in extensive biomedical applications is indicated.<\/jats:p>","DOI":"10.1002\/jemt.23553","type":"journal-article","created":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T12:31:38Z","timestamp":1596630698000},"page":"1574-1584","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["Synthesis and characterization of microbial mediated cadmium oxide nanoparticles"],"prefix":"10.1002","volume":"83","author":[{"given":"Mehrina","family":"Asghar","sequence":"first","affiliation":[{"name":"Department of Biotechnology Mohi\u2010ud\u2010Din Islamic University  Nerian Sharif Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadaf","family":"Habib","sequence":"additional","affiliation":[{"name":"School of Life Sciences Sun Yat\u2010sen University  Guangzhou Guangdong China"},{"name":"Shenzhen Key Laboratory of Southern Subtropical Plant Diversity, Fairy Lake Botanical Garden Shenzhen &amp; Chinese Academy of Sciences  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6864-2366","authenticated-orcid":false,"given":"Wajid","family":"Zaman","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajjad","family":"Hussain","sequence":"additional","affiliation":[{"name":"Henan Key Laboratory of Boron Chemistry and Advanced Energy Materials, School of Chemistry and Chemical Engineering Henan Normal University  Xinxiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huma","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Microbilogy Quaid\u2010i\u2010Azam University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1240-8949","authenticated-orcid":false,"given":"Saddam","family":"Saqib","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Mohi\u2010ud\u2010Din Islamic University  Nerian Sharif Pakistan"},{"name":"State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.11.030"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.23066"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/aoc.3506"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2019.101075"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"e5829","DOI":"10.1002\/aoc.5829","article-title":"Synthesis, characterization, DNA binding\/cleavage, and anticancer and antimicrobial activities: Nano\u2010sized co (II) and cd (II) complexes and their use as a precursor for CoO and CdO nanoparticles","volume":"2020","author":"Alothman A. 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