{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T16:59:56Z","timestamp":1773075596662,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T00:00:00Z","timestamp":1600819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003790","name":"Hiroshima University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003790","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003993","name":"Ministry of Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003993","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011821","name":"Ministry of Education \u2013 Kingdom of Saudi Arabi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011821","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In recent decades, several mass mortalities were recorded in riverine ayu (<jats:italic>Plecoglossus altivelis<\/jats:italic>) in Tokyo Metropolis, Hiroshima Prefecture, and Yamaguchi Prefecture, Japan; in these outbreaks, microbiological and pathological examinations revealed <jats:italic>Edwardsiella ictaluri<\/jats:italic> as the causative agent. In this study, histopathological findings and immunohistochemical localization of the bacteria following experimental infection of ayu were discussed. Infection experiments were performed using 44 healthy cultured ayu fingerlings using <jats:italic>E. ictaluri<\/jats:italic> isolate (H90). The fish were injected with the isolate intraperitoneally with a dose of 5.1\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> cfu\/fish, while the control fish were injected with sterile phosphate buffered saline. The fish were observed for clinical signs, with daily collection of dead fish, and isolation of bacteria from the posterior kidney was performed and confirmed to be <jats:italic>E. ictaluri<\/jats:italic> by slide agglutination using anti\u2010PH0744 serum. Daily collection of five moribund fish for necropsy and tissue specimens collection from hepatopancreas, spleen, posterior kidney, gills, brain, heart, and intestine for histopathological and immunohistochemical examination. Post\u2010mortem lesions were recorded as exophthalmia, bloody ascitis, hemorrhagic kidney and distended gallbladder, meningio\u2010encephalitis, hemorrhagic vent, and petechial hemorrhages on viscera. Histopathological examination revealed diffuse severe congestion in blood vessels and several degenerative and necrotic changes inconcurrent with positive antigenic staining by immunohistochemistry.<\/jats:p>","DOI":"10.1002\/jemt.23602","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T05:43:20Z","timestamp":1600839800000},"page":"460-470","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Pathological and immunohistochemical studies following the experimental infection of ayu (<i>Plecoglossus altivelis<\/i>) by <i>Edwardsiella ictaluri<\/i>"],"prefix":"10.1002","volume":"84","author":[{"given":"Alkhateib Y.","family":"Gaafar","sequence":"first","affiliation":[{"name":"Hydrobiology Department, Veterinary Research Division National Research Centre  Dokki Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ebtsam Sayed","family":"Hassan Abdullah","sequence":"additional","affiliation":[{"name":"Department of Aquatic Animal Medicine and Management Faculty of Veterinary Medicine, Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud Mostafa","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Department of Aquatic Animal Medicine and Management Faculty of Veterinary Medicine, Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelgayed M.","family":"Younes","sequence":"additional","affiliation":[{"name":"Hydrobiology Department, Veterinary Research Division National Research Centre  Dokki Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiro","family":"Nakai","sequence":"additional","affiliation":[{"name":"Graduate School of Biosphere Science Hiroshima University  Higashi\u2010Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-7345.1983.tb00081.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8667(1993)005<0189:POESOC>2.3.CO;2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao008113"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0044-8486(90)90149-H"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.1985.tb04018.x"},{"key":"e_1_2_10_7_1","first-page":"125","article-title":"Edwardsiella ictaluri isolated from cultured eel in Taiwan","volume":"10","author":"Chung H.","year":"1983","journal-title":"COA Fisheries Series"},{"issue":"1","key":"e_1_2_10_8_1","first-page":"33","article-title":"Edwardsiella, a new genus of Enterobacteriaceae based on a new species, E. tarda","volume":"15","author":"Ewing W.","year":"1965","journal-title":"International Bulletin of Bacteriological Nomenclature and Taxonomy"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.45.43"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.47.64"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1139\/f79-219"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/08997659.2013.782226"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-31-4-396"},{"key":"e_1_2_10_14_1","volume-title":"Microscopy, immunohistochemistry, and antigen retrieval methods: For light and electron microscopy","author":"Hayat M. 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Paper presented at the Proceedings of the 4th Global Fisheries and Aquaculture Research Conference the Egyptian International Center for Agriculture Giza Egypt 3\u20135 October2011."},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8667(1999)011<0175:ETESOC>2.0.CO;2"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.20.219"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1985.tb00961.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.43.158"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.1989.tb00322.x"},{"issue":"5","key":"e_1_2_10_27_1","first-page":"646","article-title":"Experimental Edwardsiella ictaluri infection causes mortality in white perch (Morone americana)","volume":"6","author":"Pasnik D. 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