{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T21:04:42Z","timestamp":1768338282298,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T00:00:00Z","timestamp":1605052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lobomycosis is a skin infection produced by the fungus <jats:italic>Lacazia loboi<\/jats:italic>, which mainly affects some indigenous and afro\u2010descendant populations in Tropical America. We previously reported the comparative effect of osmium tetroxide (OsO<jats:sub>4<\/jats:sub>) and ruthenium tetroxide (RuO<jats:sub>4<\/jats:sub>) in the electron microscopy (EM) of other related microorganisms. The objective of this study is to compare the effect of postfixation with OsO<jats:sub>4<\/jats:sub> and RuO<jats:sub>4<\/jats:sub> in the ultrastructure of <jats:italic>L<\/jats:italic>. <jats:italic>loboi<\/jats:italic> yeasts. Skin biopsies on patients diagnosed with lobomycosis were fixed in glutaraldehyde at 3% and postfixed in the following solutions: (a) 1% OsO<jats:sub>4<\/jats:sub>, (b) 0.2% RuO<jats:sub>4<\/jats:sub>, and (c) OsO<jats:sub>4<\/jats:sub> at 1% followed by RuO<jats:sub>4<\/jats:sub> at 0.2%. They were then processed using the conventional method for EM. Unlike OsO<jats:sub>4,<\/jats:sub> the treatment with RuO<jats:sub>4<\/jats:sub> revealed different shades of gray and electron dense bands in the cell wall and other cell components of <jats:italic>L<\/jats:italic>. <jats:italic>loboi<\/jats:italic>. The most notable finding was the presence of radial filamentous structures around the yeast, which made the image look like the sun. Postfixation with RuO<jats:sub>4<\/jats:sub> revealed ultrastructural details that had not been previously reported for <jats:italic>L loboi<\/jats:italic>. The combined use of OsO<jats:sub>4<\/jats:sub> and RuO<jats:sub>4<\/jats:sub> in EM of microorganisms with cell walls can be useful to evaluate the effect of microbicide substances.<\/jats:p>","DOI":"10.1002\/jemt.23638","type":"journal-article","created":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T13:13:57Z","timestamp":1605100437000},"page":"789-795","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative effect of osmium tetroxide and ruthenium tetroxide on <scp><i>Lacazia loboi<\/i><\/scp> ultrastructure"],"prefix":"10.1002","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7693-6206","authenticated-orcid":false,"given":"Ladys","family":"Sarmiento\u2010Lacera","sequence":"first","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular Direcci\u00f3n de Investigaci\u00f3n en Salud P\u00fablica, Instituto Nacional de Salud  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orlando","family":"Torres\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular Direcci\u00f3n de Investigaci\u00f3n en Salud P\u00fablica, Instituto Nacional de Salud  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge A.","family":"Rivera","sequence":"additional","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular Direcci\u00f3n de Investigaci\u00f3n en Salud P\u00fablica, Instituto Nacional de Salud  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerza\u00edn","family":"Rodr\u00edguez\u2010Toro","sequence":"additional","affiliation":[{"name":"Grupo de Morfolog\u00eda Celular Direcci\u00f3n de Investigaci\u00f3n en Salud P\u00fablica, Instituto Nacional de Salud  Bogot\u00e1 Colombia"},{"name":"Facultad de Medicina Universidad de la Sabana  Bogot\u00e1 Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2504.181403"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(54)80091-7"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MR.55.1.1-20.1991"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-776-1_4"},{"key":"e_1_2_10_6_1","unstructured":"Emsdiasum. 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