{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T19:31:10Z","timestamp":1785871870107,"version":"3.56.0"},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T00:00:00Z","timestamp":1622160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>N\u2010Nitrosodibutylamine (DBN) has been found in a wide variety of products because of the nitrosation of amines present in these products. An extensive series of synthesis and carcinogenicity studies of various compounds related to DBN and its metabolites have revealed that they have markedly different carcinogenic effects. The role of mitochondria in disease has been found to be expanded beyond the respiratory chain, as defects in additional mitochondrial functions and behaviors have been linked to cancer, metabolic disorders, and many neurodegenerative diseases, such as Alzheimer's, Parkinson's, and Huntington's disease (Nunnari &amp; Suomalainen (2012) Cell, 148, 1145\u20131159). The sample preparation was performed using the protocol standardized by Sophisticated Analytical Instrumentation Facility (SAIF) laboratory in NEHU. The prepared sample was then mounted and observed under the transmission electron microscope model JEM\u2010100 CC II. The micrographs obtained from transmission electron microscopy (TEM) at various magnifications 2,000, 5,000, and 12,000\u00d7 from DBN\u2010treated mice showed significant changes. Mitochondria showed variability in number, size, and shape. This significant alteration in the morphology and population of liver mitochondria observed in DBN\u2010treated animals in comparison to that of the age\u2010matched normal control mice provide a unique potential for the use of mitochondria as markers for the early detection for the treatment of liver cancer.<\/jats:p>","DOI":"10.1002\/jemt.23842","type":"journal-article","created":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T09:52:47Z","timestamp":1622195567000},"page":"2832-2836","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transmission electron microscopy of the liver mitochondria of N\u2010Nitrosodibutylamine\u2010treated mice"],"prefix":"10.1002","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8213-3063","authenticated-orcid":false,"given":"Sthiti Porna","family":"Dutta","sequence":"first","affiliation":[{"name":"The Assam Royal Global University  Guwahati India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kundal","family":"Neog","sequence":"additional","affiliation":[{"name":"Assam down town University  Guwahati India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anis","family":"Alam","sequence":"additional","affiliation":[{"name":"North Eastern Hill University  Shillong India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03381"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004200050217"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.200800024"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.44.2.278"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-1-9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M503062200"},{"key":"e_1_2_8_8_1","first-page":"6674","article-title":"The bioenergetic signature of cancer: A marker of tumor progression","volume":"62","author":"Cuveza J. M.","year":"2002","journal-title":"Cancer Research"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2009.01061.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.02.002"},{"key":"e_1_2_8_11_1","volume-title":"Monographs on the evaluation of the carcinogenic risk of chemicals to humans","author":"IARC","year":"1972"},{"key":"e_1_2_8_12_1","first-page":"365","volume-title":"IARC monographs on the evaluation of the carcinogen risk of chemicals to humans: Some N\u2010Nitroso compounds","author":"IARC","year":"1978"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601184"},{"issue":"2","key":"e_1_2_8_14_1","first-page":"126","article-title":"DNA damage in different organs of rats treated with N\u2010nitrosodibutylamine","volume":"20","author":"Kornuta N. A.","year":"1998","journal-title":"Experimental Oncology"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.35.443"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2002.tb02583.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.02.035"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209602"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401252"},{"key":"e_1_2_8_20_1","first-page":"1486","article-title":"Characteristics of mitochondria isolated by rate zonal centrifugation from normal liver and novikoff hepatomas","volume":"40","author":"Stocco D. M.","year":"1980","journal-title":"Cancer Research"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvf113"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.23842","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jemt.23842","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.23842","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T18:32:42Z","timestamp":1760639562000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.23842"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,28]]},"references-count":20,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/jemt.23842"],"URL":"https:\/\/doi.org\/10.1002\/jemt.23842","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,28]]},"assertion":[{"value":"2020-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}