{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:38:53Z","timestamp":1788536333082,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T00:00:00Z","timestamp":1630281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Buddleja asiatica<\/jats:italic> commonly known as \u201cBooi\u201d is a large deciduous shrub belongs to family Scrophulariaceae, traditionally used as antipyretic, analgesic, hypotensive, antimicrobial, anti\u2010inflammatory, and antifungal agent recorded from essential oil obtained from leaves. The literature revealed that the plant has been widely used for many other purposes. The said plant has been analyzed through pharmacognostic techniques such as morphological, microscopic, and physio\u2010chemical evaluations to keep the originality of the plant and to avoid adulteration. Morphologically, the plant is evergreen shrub, while organoleptic studies showed that the leaf has glabrous upper surface and tomentose lower surface, with slightly bitter taste and aromatic odor. Anatomically, the leaf showed typical dicot histological differentiation with hypostomatic nature having highest frequency (90%) of actinocytic stomata. The average stomatal number and stomatal index were 336\u2009\u00b1\u200939.5 and 30.01\u2009\u00b1\u20092.34, respectively. The palisade cell ratio, vein termination, and vein islet number were 9.2\u2009\u00b1\u20090.72, 10.2\u2009\u00b1\u20093.1, and 10.3\u2009\u00b1\u20093.3, respectively. Various tissue fragments have been observed during powdered drug analysis of the leaf. Preliminary phytochemical screening confirmed the presence of proteins, phenols, alkaloids, saponins, flavonoids, tannins, and glycosides. Fluorescence analysis in ordinary day light and under UV light along with extractive values was also analyzed. The above\u2010mentioned studies that have been reported, for the first time, for the said plant may be significant to establish the pharmacognostic and phytochemical standards of the said species.<\/jats:p>","DOI":"10.1002\/jemt.23924","type":"journal-article","created":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T03:23:30Z","timestamp":1630293810000},"page":"510-520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Phytochemical and pharmacognostic studies of <scp><i>Buddleja asiatica<\/i><\/scp> leaves"],"prefix":"10.1002","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9458-3873","authenticated-orcid":false,"given":"Muhammad","family":"Nafees","sequence":"first","affiliation":[{"name":"Department of Botany University of Peshawar  Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Barkatullah","sequence":"additional","affiliation":[{"name":"Department of Botany Islamia College University  Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Botany University of Peshawar  Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazia","family":"Ikram","sequence":"additional","affiliation":[{"name":"Department of Botany University of Peshawar  Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,30]]},"reference":[{"issue":"3","key":"e_1_2_6_2_1","first-page":"165","article-title":"Pharmacognosy, phytochemistry, pharmacology and clinical applications of Taraxacum officinale","volume":"10","author":"Ajmire P. V.","year":"2021","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"issue":"4","key":"e_1_2_6_3_1","first-page":"1737","article-title":"Biological screening of different root extracts of Euphorbia wallichii","volume":"41","author":"Ali I. N.","year":"2009","journal-title":"Pakistan Journal of Botany"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2020.11.013"},{"issue":"1","key":"e_1_2_6_5_1","first-page":"10","article-title":"The chemical constituents and pharmacological effects of Chenopodium album","volume":"5","author":"Al\u2010Snafi A. E.","year":"2015","journal-title":"International Journal of Pharmacological Screening Methods"},{"key":"e_1_2_6_6_1","first-page":"82","article-title":"Pharmacognostic evaluation of the stem bark of Balanitesa egyptica","volume":"50","author":"Ansari M. M.","year":"2006","journal-title":"Hamdard Medicus"},{"issue":"2","key":"e_1_2_6_7_1","first-page":"145","article-title":"Quality control, HPTLC analysis, antioxidant and antimicrobial activity of hydroalcoholic extract of roots of qust (Saussurea lappa, CB Clarke)","volume":"36","author":"Ansari S.","year":"2021","journal-title":"Drug Metabolism and Personalized Therapy"},{"issue":"1","key":"e_1_2_6_8_1","first-page":"1","article-title":"Pharmacognostic investigation on leaves of Citrus maxima (Rutaceae)","volume":"1","author":"Aswini K.","year":"2012","journal-title":"Journal of Pharmaceutical Sciences"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jarmap.2018.11.003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.5958\/0974-360X.2021.00300.0"},{"issue":"1","key":"e_1_2_6_11_1","first-page":"58","article-title":"Pharmacognostic investigation of Baptisia tinctoria","volume":"2","author":"Bansal S. K.","year":"2012","journal-title":"International Journal of Recent Advances in Pharmaceutical Research"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12210-020-00911-z"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.5958\/0974-360X.2020.00772.6"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(01)02050-7"},{"key":"e_1_2_6_15_1","first-page":"13884","article-title":"Pharmacognostic evaluation, estimation of phenolic, flavonoid composition and antioxidant activity of Aspidopterys indica (Willd.) W. Theob: An endemic plant to peninsular India","volume":"25","author":"Chandrika P. U.","year":"2021","journal-title":"Annals of the Romanian Society for Cell Biology"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaim.2017.09.008"},{"issue":"1","key":"e_1_2_6_17_1","first-page":"7","article-title":"Pharmacognostic characteristics and mutagenic studies of Alstonia boonei De Wild","volume":"7","author":"Dodoo K.","year":"2020","journal-title":"Research Journal of Pharmacognosy"},{"key":"e_1_2_6_18_1","first-page":"553","volume-title":"Trease and Evans pharmacognosy","author":"Evans W. C.","year":"2009"},{"issue":"31","key":"e_1_2_6_19_1","first-page":"1","article-title":"Cannabis and its secondary metabolites: Their use as therapeutic drugs, toxicological aspects and analytical determination","volume":"6","author":"Goncalves J.","year":"2019","journal-title":"Medicine"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221-1691(12)60065-3"},{"key":"e_1_2_6_21_1","first-page":"33","volume-title":"Phytochemical methods","author":"Harborne J. B.","year":"1998"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/10496475.2019.1666769"},{"issue":"11","key":"e_1_2_6_23_1","first-page":"89","article-title":"Pharmacognostical and physicochemical evaluation of Pterospermum suberifolium Linn leaves","volume":"2","author":"Jain V. K.","year":"2011","journal-title":"International Journal of Pharmaceutical Research and Development"},{"issue":"3","key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"254","DOI":"10.4103\/japtr.JAPTR_238_21","article-title":"Phytochemical screening, antimalarial activities, and genetic relationship of 16 indigenous Thai Asteraceae medicinal plants: A combinatorial approach using phylogeny and ethnobotanical bioprospecting in antimalarial drug discovery","volume":"12","author":"Liana D.","year":"2021","journal-title":"Journal of Advanced Pharmaceutical Technology & Research"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21101374"},{"issue":"6","key":"e_1_2_6_26_1","first-page":"3705","article-title":"Pharmacognostic studies on fresh mature leaves of Holoptelea integrifolia (Roxb) Planch","volume":"42","author":"Mahmud S.","year":"2010","journal-title":"Pakistan Journal of Botany"},{"issue":"3","key":"e_1_2_6_27_1","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.22271\/phyto.2020.v9.i3q.11428","article-title":"Phytochemical screening and pharmacological update on Kabasura Kudineer Choornam and Nilavembu Kudineer Choornam","volume":"9","author":"Mekala P.","year":"2020","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"issue":"2","key":"e_1_2_6_28_1","first-page":"94","article-title":"Phytochemical and pharmacognostic analysis of Ficus thonningii Blume leaves for monograph development","volume":"4","author":"Omoregie E. H.","year":"2015","journal-title":"International Journal of Basic and Applied Sciences"},{"issue":"1","key":"e_1_2_6_29_1","first-page":"50","article-title":"Preliminary pharmacognostic evaluations and phytochemical studies on roots of Mimosa pudica (Lajvanti)","volume":"1","author":"Pande M.","year":"2010","journal-title":"International Journal of Pharmaceutical Sciences Review and Research"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.5958\/0975-4385.2020.00014.X"},{"issue":"4","key":"e_1_2_6_31_1","first-page":"274","article-title":"Pharmacognostic studies of the oil, aerial parts, pulp and feel of Citrullus colocynthis","volume":"24","author":"Saleem M.","year":"2010","journal-title":"Journal of the Chemical Society of Pakistan"},{"issue":"3","key":"e_1_2_6_32_1","first-page":"337","article-title":"Pharmacognostic and phytochemical studies of Ocimum americanum","volume":"3","author":"Sarma D. S. K.","year":"2011","journal-title":"Journal of Chemical and Pharmaceutical Research"},{"issue":"1","key":"e_1_2_6_33_1","first-page":"121","article-title":"Pharmacognostic studies of the Lagenaria siceraria (Molina) Standley","volume":"2","author":"Shah B. N.","year":"2010","journal-title":"International Journal of Pharmaceutical Technology"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.22271\/chemi.2020.v8.i2i.8834"},{"issue":"2","key":"e_1_2_6_35_1","first-page":"445","article-title":"Phytochemical screening and antibacterial activity of lemongrass (Cymbopogon citratus) leaves essential oil","volume":"10","author":"Shendurse A. M.","year":"2021","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"issue":"3","key":"e_1_2_6_36_1","first-page":"1","article-title":"Pharmacognostic studies of the leaves of Tinospora Cordifolia","volume":"1","author":"Soni H.","year":"2011","journal-title":"Journal of Pharmacognosy and Herbal Formulations"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287394.2021.1875345"},{"key":"e_1_2_6_38_1","first-page":"572","volume-title":"Text book of pharmacognosy","author":"Wallis T. E.","year":"2009"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.683265"},{"key":"e_1_2_6_40_1","first-page":"5","article-title":"Pharmacognostic, physicochemical and phytochemical studies on Carica papaya Linn. Leaves","volume":"20","author":"Zunjar V.","year":"2015","journal-title":"Pharmacognosy Journal"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.23924","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jemt.23924","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.23924","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:20:32Z","timestamp":1760631632000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.23924"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,30]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/jemt.23924"],"URL":"https:\/\/doi.org\/10.1002\/jemt.23924","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,30]]},"assertion":[{"value":"2021-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}