{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:47:59Z","timestamp":1760986079195,"version":"build-2065373602"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,3]],"date-time":"2021-12-03T00:00:00Z","timestamp":1638489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["AR 052353","AR 069047"],"award-info":[{"award-number":["AR 052353","AR 069047"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This dual\u2010modality microscopic imaging study quantifies the interface region between the noncalcified cartilage and the subchondral bone plate, which includes the deep portion of the noncalcified articular cartilage and the zone of calcified cartilage (ZCC). This interface region is typically not visible in routine MRI but becomes visible in MRI with the application of an ultra\u2010short echo time (UTE) sequence. A number of cartilage\u2010bone blocks from a well\u2010documented canine humeral head were harvested for imaging by microscopic MRI (\u03bcMRI) and PLM (polarized light microscopy). In \u03bcMRI, T2 anisotropic images were acquired by 2D gradient\u2010echo, magnetization\u2010prepared spin\u2010echo and UTE sequences at the 0\u00b0 and 55\u00b0 (the magic angle) orientations at 11.7\u00a0\u03bcm\/pixel resolution. In PLM, quantitative optical retardation (nm) and collagen orientation (\u00b0) were mapped from the thin sections from the same \u03bcMRI specimens at 0.5\u20132\u00a0\u03bcm pixel resolutions. The orientational and organizational architecture of the collagen matrix in this interface region was quantified and correlated between the complementary imaging. The magic angle effect as seen in the noncalcified cartilage was statistically confirmed in ZCC in \u03bcMRI, which was further supported by quantitative PLM. With an enhanced understanding of the tissue properties in this important interface region, it will potentially be possible to monitor the changes of this tissue region which is instrumental to the initiation and development of osteoarthritis and other joint diseases.<\/jats:p>","DOI":"10.1002\/jemt.24011","type":"journal-article","created":{"date-parts":[[2021,12,3]],"date-time":"2021-12-03T03:13:39Z","timestamp":1638501219000},"page":"1483-1493","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The interface region between articular cartilage and bone by <scp>\u03bcMRI<\/scp> and <scp>PLM<\/scp> at microscopic resolutions"],"prefix":"10.1002","volume":"85","author":[{"given":"Farid","family":"Badar","sequence":"first","affiliation":[{"name":"Department of Physics and Center for Biomedical Research Oakland University  Rochester Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4653-1862","authenticated-orcid":false,"given":"Yang","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Physics and Center for Biomedical Research Oakland University  Rochester Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,3]]},"reference":[{"key":"e_1_2_8_2_1","article-title":"Early alterations of subchondral bone in the rat anterior cruciate ligament transection model of osteoarthritis","author":"Aizah N.","year":"2019","journal-title":"Cartilage"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2003.011783"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mri.2021.01.002"},{"issue":"2","key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s40134-013-0035-7","article-title":"UTE MRI of the osteochondral junction","volume":"2","author":"Bae W. 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