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This study aimed to produce functionalized and biocompatible silver nanoparticles (AgNPs) using cellular extracts of endophytic <jats:italic>Fusarium oxysporum<\/jats:italic>\u2010NFW16 isolated from <jats:italic>Taxus fauna<\/jats:italic> and evaluate its antibacterial potential. Under optimized reaction conditions, well\u2010dispersed and extremely stable AgNPs were synthesized in 1\u2009hr. AgNPs were characterized through UV\u2013visible spectrophotometry (at 423\u2009nm), and scanning electron microscopy, energy dispersive X\u2010ray spectroscopy, X\u2010ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The obtained AgNPs were spherical, monodispersed, and size was ~30\u201336.1\u00a0nm. Strong peaks of XRD (311), (220), (200), and (111) matched to silver plane's diffraction facets. FTIR spectra at 1,650, 2,950, and 1,400\u2009cm<jats:sup>\u22121<\/jats:sup> confirmed the capping of AgNPs with phenolic compounds and compounds having primary amines. The AgNPs showed 100\u2009\u03bcg\/ml of minimum inhibitory concentration against methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA). In addition, AgNPs showed a synergistic effect with both vancomycin and ciprofloxacin against MRSA (25%), <jats:italic>Pseudomonas aeruginosa<\/jats:italic> (50%), and pus isolated <jats:italic>Escherichia coli<\/jats:italic> (50%). Moreover, AgNPs impregnated cotton and bandage showed in vitro antibacterial potential against American Type Culture Collection and skin\u2010associated clinical pathogenic bacteria. Findings showed that endophytic fungi are the potential source for AgNPs synthesis that are effective against multidrug\u2010resistant bacteria and the development of antimicrobial textile finishes.<\/jats:p>","DOI":"10.1002\/jemt.24018","type":"journal-article","created":{"date-parts":[[2021,12,10]],"date-time":"2021-12-10T01:58:06Z","timestamp":1639101486000},"page":"1568-1579","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Biosynthesis of silver nanoparticles using endophytic <i>Fusarium oxysporum<\/i> strain <scp>NFW16<\/scp> and their in vitro antibacterial potential"],"prefix":"10.1002","volume":"85","author":[{"given":"Nikhat","family":"Ilahi","sequence":"first","affiliation":[{"name":"Department of Microbiology, Applied, Environmental and Geomicrobiology Laboratory Quaid\u2010i\u2010Azam University  Islamabad 45320 Pakistan"},{"name":"School of Life Sciences State Key Laboratory of Grassland Agro\u2010Ecosystems, Lanzhou University  Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Haleem","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Applied, Environmental and Geomicrobiology Laboratory Quaid\u2010i\u2010Azam University  Islamabad 45320 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajid","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Applied, Environmental and Geomicrobiology Laboratory Quaid\u2010i\u2010Azam University  Islamabad 45320 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nighat","family":"Fatima","sequence":"additional","affiliation":[{"name":"Department of Biosciences Comsat Institute of Technology  Abbottabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1622-0484","authenticated-orcid":false,"given":"Wasim","family":"Sajjad","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cryospheric Science Northwest Institute of Eco\u2010Environment and Resources, University of Chinese Academy of Sciences  Lanzhou 730000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abubakar","family":"Sideeq","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Applied, Environmental and Geomicrobiology Laboratory Quaid\u2010i\u2010Azam University  Islamabad 45320 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safia","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Applied, Environmental and Geomicrobiology Laboratory Quaid\u2010i\u2010Azam University  Islamabad 45320 Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12668-017-0413-3"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0927-7765(02)00174-1"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2015.02.007"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2015.05.009"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2015.09.009"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.materresbull.2007.06.020"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25010112"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/246012"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1088\/1757-899X\/290\/1\/012002"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13204-021-02040-x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S231340"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtcomm.2020.101385"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/22297928.2021.1883477"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmau.2014.10.004"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01090"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2007.022"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-3155-3-8"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2014.02.024"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2015.04.018"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.06.039"},{"key":"e_1_2_10_22_1","unstructured":"Fatima N.(2013). 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