{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T22:09:18Z","timestamp":1785449358590,"version":"3.56.0"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T00:00:00Z","timestamp":1640563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Studies seeking control methods for infestation of <jats:italic>Riphicephalus sanguineus<\/jats:italic> sensu lato (s.l.) ticks (dog ticks) have been carried out in order to minimize damage to both the tick's hosts and the environment, the latter due to the misuse of acaricide products. In this regard, carvacrol has been used as a natural alternative against ticks as it displays several properties including acaricidal. In this context, the present study aimed to evaluate the ultramorphology and morphohistochemistry of the integument of semi\u2010engorged <jats:italic>R. sanguineus<\/jats:italic> s.l. females exposed to different carvacrol concentrations. The findings indicate that the integument surface of females exposed to the highest carvacrol concentration (25\u2009\u03bcl\/ml) became wrinkled, suggesting dehydration or the result of integument cuticular and epithelial layer disorganization in response to the toxic product. Morphohistochemical integument layer alterations were more significant and intense in females exposed to the highest carvacrol concentration (25\u2009\u03bcl\/ml), confirming dose\u2010dependent carvacrol action. Among other cell and tissue alterations, changes in epithelial cell shape, size, and arrangement (epidermal layer) were noted, alongside altered and pyknotic\u2010shaped nuclei, suggesting a death process for these cells. This epithelium changed from simple cubic to stratified, also in response to the presence of the evaluated bioactive compound. Thus, the findings reported herein demonstrate that carvacrol may be an alternative for an efficient and more sustainable tick control in the near future.<\/jats:p>","DOI":"10.1002\/jemt.24038","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T01:46:43Z","timestamp":1640656003000},"page":"1784-1790","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The bioactive compound carvacrol as a potential acaricide: An assessment of its effects on the integument of female <i>Rhipicephalus sanguineus<\/i> sensu lato ticks"],"prefix":"10.1002","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2605-0420","authenticated-orcid":false,"given":"Jos\u00e9 Ribamar","family":"Lima\u2010de\u2010Souza","sequence":"first","affiliation":[{"name":"Center for Biological and Natural Sciences (CCBN) Federal University of Acre (UFAC)  Rio Branco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2695-2101","authenticated-orcid":false,"given":"Patr\u00edcia Rosa","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Biosciences Postgraduate Program, Multidisciplinary Institute for Health Federal University of Bahia (UFBA)  Vit\u00f3ria da Conquista\/BA Brazil"},{"name":"Chemistry Postgraduate Program, Exact and Technology Sciences Center Federal University of Maranh\u00e3o (UFMA)  S\u00e3o Lu\u00eds\/MA Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8712-2630","authenticated-orcid":false,"given":"Lu\u00eds Adriano","family":"Anholeto","sequence":"additional","affiliation":[{"name":"Department of Biology, Institute of Biosciences S\u00e3o Paulo State University\u2010UNESP  Rio Claro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds Fernando","family":"Sodelli","sequence":"additional","affiliation":[{"name":"Department of Biology, Institute of Biosciences S\u00e3o Paulo State University\u2010UNESP  Rio Claro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan Roberto Fernandes","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Biology, Institute of Biosciences S\u00e3o Paulo State University\u2010UNESP  Rio Claro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Neodini","family":"Remedio","sequence":"additional","affiliation":[{"name":"Department of Health Sciences Federal University of Lavras  Lavras Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9418-8934","authenticated-orcid":false,"given":"Maria Izabel","family":"Camargo\u2010Mathias","sequence":"additional","affiliation":[{"name":"Department of Biology, Institute of Biosciences S\u00e3o Paulo State University\u2010UNESP  Rio Claro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.03.006"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2011.12.002"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2016.09.018"},{"key":"e_1_2_10_5_1","first-page":"61","article-title":"Rhipicephalus sanguineus tick in Brazil: Feeding and reproductive aspects under laboratorial conditions","volume":"4","author":"Bechara G. 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