{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:36:57Z","timestamp":1790221017234,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,5,12]],"date-time":"2022-05-12T00:00:00Z","timestamp":1652313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The present study described the full morphology of the duodenum of the Japanese quail during the embryonic stage from 3rd day of incubation till hatching using the light and electron (scanning and transmission) microscope. The specimens were collected, analyzed and described anatomically, morphometrically and microscopically. The first recognition of the prospective duodenum was at the 4th day of incubation and developed continuously by age progression. The prospective duodenum consisted of a flat pseudostratified epithelium, mesenchyme and covering mesothelium. On day 8th of incubation, the epithelium developed three evaginations lead to formation three previllous ridges protruding inside the duodenal lumen, which later at the 9th day differentiated into numbers of projections; villi. On the 9th day, the epithelium lined the villi transformed into a simple columnar type, the duodenal villi appeared as pyramidal\u2010shaped projections, had wide base and narrow apex and by age progression, the duodenal villi went through changes in number, size and shape. On hatching day, the duodenal epithelium consisted of enterocytes interspersed with secretory goblet cells, which stained positive for both Periodic Acid Schiff (PAS) and Alcian blue AB and represented filled with metachromatic granules. The muscular wall started as mesenchymal condensation on the 6th day then differentiated into the circular smooth muscle layer on the 9th day of incubation. Giving detailed information about the morphological development of the duodenum during the incubation period of quail embryo helps in reaching a satisfactory explanation about how the duodenum plays a vital role in digestion, absorption and immunity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Studying the quail duodenum can be used as a model for understanding the mammalian duodenum.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Understanding the duodenal structure and its function is the best way to maximize the efficiency of the production of the livestock through giving the best type of diet.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Duodenum plays a vital role in digestion through the digestible secretions, absorption of nutrients, and immunity against invaders.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Duodenum is the spot where the food digestion mostly occurs.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24146","type":"journal-article","created":{"date-parts":[[2022,5,12]],"date-time":"2022-05-12T10:39:31Z","timestamp":1652351971000},"page":"2965-2983","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Tracking the developmental events in the duodenum of the quail embryo: Using light and electron microscope"],"prefix":"10.1002","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6822-1454","authenticated-orcid":false,"given":"Fatma","family":"Abdelhakeem","sequence":"first","affiliation":[{"name":"Department of Pathophysiology, School of Basic Medical Sciences Zhengzhou University  Zhengzhou Henan People's Republic of China"},{"name":"Department of Anatomy and Embryology South Valley University  Qena Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salma A.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology South Valley University  Qena Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Abdelsabour\u2010Khalaf","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology South Valley University  Qena Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5641-9260","authenticated-orcid":false,"given":"Fatma A.","family":"Madkour","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology South Valley University  Qena Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,12]]},"reference":[{"issue":"2","key":"e_1_2_10_2_1","first-page":"103","article-title":"Histomorphological studies on the small intestine of Japanese quail (Coturnix coturnix japonica)","volume":"24","author":"Ahmad J. M.","year":"2012","journal-title":"IJAVA"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2009.01173.x"},{"issue":"1","key":"e_1_2_10_4_1","first-page":"852","article-title":"HISTO\u2010morphometric and histochemical comparison of the duodenum between goat (Capra hircus) and Gazelle (Gazella subgutturosa)","volume":"20","author":"Al\u2010Mansour N. A.","year":"2020","journal-title":"Plant Archives"},{"key":"e_1_2_10_5_1","first-page":"593","volume-title":"Theory and practice of histological techniques","author":"Bancroft J.","year":"2002"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0540155"},{"key":"e_1_2_10_7_1","volume-title":"Atlas of chick development","author":"Bellairs R.","year":"2005"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2017.01.011"},{"issue":"3","key":"e_1_2_10_9_1","first-page":"403","article-title":"Intestinal development: I. Morphogenesis of the villi and musculature","volume":"6","author":"Coulombre A. J.","year":"1958","journal-title":"Journal of Embryology and Experimental Morphology"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pey492"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.23216"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2011-02091"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.23794"},{"key":"e_1_2_10_14_1","volume-title":"Morphogenesis of the gastrointestinal tract of helmeted Guinea fowl (Numida meleagris) at pre and post\u2010hatch","author":"Gosomji I. J","year":"2014"},{"key":"e_1_2_10_15_1","volume-title":"Manual of histologic staining methods of the armed forces Institute of Pathology","author":"Luna L. G.","year":"1968"},{"issue":"1","key":"e_1_2_10_16_1","first-page":"42","article-title":"Post\u2010hatching development of ventriculus in muscovy duck: Light and electron microscopic study","volume":"12","author":"Madkour F.","year":"2022","journal-title":"Journal of Advanced Veterinary Research"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.23884"},{"key":"e_1_2_10_18_1","first-page":"408","volume-title":"The Japanese quail","author":"Mizutani M.","year":"2003"},{"key":"e_1_2_10_19_1","first-page":"27","article-title":"Morphological characterization of the laryngeal mound of the Egyptian geese","volume":"22","author":"Mohamed S. A.","year":"2018","journal-title":"Ratio"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3329\/jbau.v10i1.12096"},{"key":"e_1_2_10_21_1","unstructured":"Ravindran V.(2003).Development of digestive function in neonatal poultry: Physiological limitations and potential. In: The proceedings of the 15th annual Australian poultry science symposium Sydney Australia."},{"key":"e_1_2_10_22_1","first-page":"429","article-title":"The digestive system","volume":"1","author":"Romanoff A.","year":"1960","journal-title":"The Avian Embryo"},{"issue":"2","key":"e_1_2_10_23_1","first-page":"639","article-title":"Histo\u2010morphological study of immune cells in caecum of different poultry birds","volume":"10","author":"Senapati M.","year":"2015","journal-title":"The Bioscan"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1079\/WPS20010030"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12565-015-0318-6"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-86503-9"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pey415"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071669608417837"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/82.2.320"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3923\/ijps.2011.680.684"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/09064702.2012.676061"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2018.06.006"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/83.11.1868"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJB11.4014"},{"issue":"3","key":"e_1_2_10_35_1","first-page":"253","article-title":"Anatomical, histological and histochemical adaptations of the avian alimentary canal to their food habits: I\u2010Coturnix coturnix","volume":"9","author":"Zaher M.","year":"2012","journal-title":"Life Science Journal"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24146","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jemt.24146","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24146","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:03:12Z","timestamp":1760641392000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.24146"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,12]]},"references-count":34,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1002\/jemt.24146"],"URL":"https:\/\/doi.org\/10.1002\/jemt.24146","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,12]]},"assertion":[{"value":"2022-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}