{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T03:00:19Z","timestamp":1781233219208,"version":"3.54.1"},"reference-count":13,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,5,12]],"date-time":"2022-05-12T00:00:00Z","timestamp":1652313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The study deals with the LM and SEM (light microscopy and scanning electron microscopy) of <jats:italic>Datura stramonium<\/jats:italic> L. extract and to evaluate its phytochemical, antimicrobial, and antioxidant activities. The plant sample collected from different Lahore region was dried under shade. The plant extract was prepared using different solvents (n\u2010hexane, chloroform, ethyl acetate, and methanol). Phytochemical screening performed qualitatively for alkaloids, carbohydrates, flavonoids, saponins, and tannins. Radical scavenging potential of plant extracts was estimated and ethyl acetate extract exhibited maximum percentage scavenging ability of 92.2\u2009\u00b1\u20090.09. Total antioxidant capacity of different extracts was measured by phosphomolybdate method. The ethyl acetate and methanolic extracts showed maximum values of 72.3\u2009\u00b1\u20090.08% and 79\u2009\u00b1\u20090.15% with respect to gallic acid. Total phenolic contents were determined through Folin\u2010Ciocalteau method and highest value of 495.2\u2009\u00b1\u20093.4 was obtained by methanolic extract. DPPH\u2010TLC autography was used for the visual detection of antioxidant compounds in different plant extracts. Antibacterial activity of plant extract was measured by disc diffusion method against microorganisms named <jats:italic>Bacillus licheniformis<\/jats:italic>, <jats:italic>Bacillus subtilis<\/jats:italic> (Gram positive), and <jats:italic>Salmonella typhimurium<\/jats:italic>, and <jats:italic>Proteus mirabilis<\/jats:italic> (Gram negative). Because of the vast medicinal importance of <jats:italic>D. stramonium<\/jats:italic> L. it would be beneficial to study LM and SEM for correct identification of plant and to determine phytochemicals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>LM and SEM (light microscopy and scanning electron microscopy) of <jats:italic>Datura stramonium<\/jats:italic> L. was studied for the exact identification of plant.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>To evaluate the biological activities of pharmaceutically important plant.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Antioxidant and antimicrobial activity were determined.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:italic>D. stramonium<\/jats:italic> L. proved to be medicinally important plant.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24148","type":"journal-article","created":{"date-parts":[[2022,5,12]],"date-time":"2022-05-12T10:43:37Z","timestamp":1652352217000},"page":"2997-3004","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Light and scanning electron microscopy of <i>Datura stramonium<\/i> L. extract and its biological applications"],"prefix":"10.1002","volume":"85","author":[{"given":"Shafiq Ur","family":"Rehman","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Central Punjab  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadeem","family":"Ullah","sequence":"additional","affiliation":[{"name":"Department of Botany University of Central Punjab  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2000-3079","authenticated-orcid":false,"given":"Mehwish","family":"Jaffer","sequence":"additional","affiliation":[{"name":"Department of Botany University of Central Punjab  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riffat","family":"Zaidi","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Central Punjab  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hina","family":"Anwar","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Central Punjab  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3329\/dujps.v14i1.23733"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(98)00055-5"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2013.07.002"},{"issue":"5","key":"e_1_2_6_5_1","first-page":"69","article-title":"Importance of pharmacognostic study of medicinal plants: An overview","volume":"2","author":"Chanda S.","year":"2014","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"key":"e_1_2_6_6_1","first-page":"512","article-title":"Chemical constituents and pharmacological properties of Datura stramonium (thorn apple)\u2014A review","volume":"8","author":"Cornelius G.","year":"2019","journal-title":"International Journal of Engineering Research & Technology (IJERT)"},{"issue":"1","key":"e_1_2_6_7_1","first-page":"1","article-title":"Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves","volume":"6","author":"Islam E.","year":"2013","journal-title":"BMC Research Notes"},{"key":"e_1_2_6_8_1","first-page":"540","article-title":"Characterization, purification and identification of some alkaloids in Datura stramonium","volume":"24","author":"Jamal P.","year":"2017","journal-title":"International Food Research Journal"},{"key":"e_1_2_6_9_1","volume-title":"Proceedings of the 10th International Rapeseed Congress","author":"Peiwu L.","year":"1999"},{"key":"e_1_2_6_10_1","first-page":"123","article-title":"Phytochemistry, pharmacological and traditional uses of Datura stramonium L","volume":"2","author":"Sayyed A.","year":"2014","journal-title":"Research & Reviews: Journal of Pharmacognosy and Phytochemistry"},{"issue":"3","key":"e_1_2_6_11_1","first-page":"143","article-title":"Datura stramonium: An overview of its phytochemistry and pharmacognosy","volume":"5","author":"Singh L. R.","year":"2013","journal-title":"Research Journal of Pharmacognosy and Phytochemistry"},{"issue":"2","key":"e_1_2_6_12_1","first-page":"83","article-title":"Phytochemical analysis, antibacterial and antioxidant activity of leaf extract of Datura stramonium","volume":"1","author":"Sreenivasa S.","year":"2012","journal-title":"International Journal of Science Research"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-017-1751-y"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8178294"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24148","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jemt.24148","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24148","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:02:46Z","timestamp":1760641366000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.24148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,12]]},"references-count":13,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1002\/jemt.24148"],"URL":"https:\/\/doi.org\/10.1002\/jemt.24148","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,12]]},"assertion":[{"value":"2022-01-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}