{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:49:42Z","timestamp":1792594182749,"version":"4.5.0"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T00:00:00Z","timestamp":1656288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to assess different approaches for bone healing evaluation on histological images and to introduce a new automatic evaluation method based on segmentation with distinct thresholds. We evaluated the hyperbaric oxygen therapy (HBO) effects on bone repair in type 1 diabetes mellitus rats. Twelve animals were divided into four groups (<jats:italic>n<\/jats:italic>\u00a0=\u00a03): non\u2010diabetic, non\u2010diabetic\u2009+\u2009HBO, diabetic, and diabetic\u2009+\u2009HBO. Diabetes was induced by intravenous administration of streptozotocin (50\u2009mg\/kg). Bone defects were created in femurs and HBO was immediately started at one session\/day. After 7\u2009days, the animals were euthanized, femurs were removed, demineralized, and embedded in paraffin. Histological sections were stained with hematoxylin and eosin (HE) and Mallory's trichrome (MT), and evaluated using three approaches: (1) conventional histomorphometric analysis (HE images) using a 144\u2010point grid to quantify the bone matrix; (2) a semi\u2010automatic method based on bone matrix segmentation to assess the bone matrix percentage (MT images); and (3) automatic approach, with the creation of a plug\u2010in for ImageJ software. The time required to perform the analysis in each method was measured and subjected to Bland\u2013Altman statistical analysis. All three methods were satisfactory for measuring bone formation and were not statistically different. The automatic approach reduced the working time compared to visual grid and semi\u2010automated method (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.01). Although histological evaluation of bone healing was performed successfully using all three methods, the novel automatic approach significantly shortened the time required for analysis and had high accuracy.<\/jats:p>","DOI":"10.1002\/jemt.24188","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T05:05:47Z","timestamp":1656306347000},"page":"3339-3346","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Automated bone healing evaluation: New approach to histomorphometric analysis"],"prefix":"10.1002","volume":"85","author":[{"given":"Camila Rodrigues Borges","family":"Linhares","sequence":"first","affiliation":[{"name":"Dentistry Department Federal University of Uberl\u00e2ndia  Uberl\u00e2ndia Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9511-5078","authenticated-orcid":false,"given":"Gustavo Davi","family":"Rabelo","sequence":"additional","affiliation":[{"name":"Dentistry Department Federal University of Santa Catarina  Florian\u00f3polis Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0089-3772","authenticated-orcid":false,"given":"Pedro Henrique Justino Oliveira","family":"Limirio","sequence":"additional","affiliation":[{"name":"Dentistry Department Federal University of Uberl\u00e2ndia  Uberl\u00e2ndia Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessyca Figueira","family":"Ven\u00e2ncio","sequence":"additional","affiliation":[{"name":"Dentistry Department Federal University of Uberl\u00e2ndia  Uberl\u00e2ndia Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor Gon\u00e7alves","family":"Ribeiro Silva","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering Federal University of Uberl\u00e2ndia  Uberl\u00e2ndia Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5509-8138","authenticated-orcid":false,"given":"Paula","family":"Dechichi","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Histology and Embryology, Biomedical Science Institute Federal University of Uberl\u00e2ndia  Uberl\u00e2ndia Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,27]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-015-1752-3"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2018.1434166"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.2337\/db11-0516"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-013-8437-4"},{"key":"e_1_2_12_6_1","volume-title":"Digital image processing","author":"Gonzalez R. 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