{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:09:10Z","timestamp":1788959350842,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T00:00:00Z","timestamp":1656979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Electrospinning technique became a very common and effective method to fabricate nonwoven films with a large surface area for different purposes especially in the biomedical field. Antimicrobial submicron fibrous films based on polyvinylpyrrolidone (PVP) doped with silver nanoparticles (Ag\u2010NPs), Zinc Oxide (ZnO), and (Ag\u2010NPS\/ZnO), were successfully fabricated via the electrospinning technique. The morphology and the elemental configurations of the as\u2010prepared films were studied by using scanning electron microscope with EDX. While the phase formation and crystal structures were determined by using XRD analysis. The antibacterial effect was investigated against one of the most common Gram\u2010negative bacteria <jats:italic>Klebsiella pneumoniae<\/jats:italic> by using a modified Kirby\u2013Bauer disc diffusion method. The results showed that the doping nanoparticles were uniformly loaded on the surface of the fabricated film fibers. By using Scherrer equation the calculated average crystallite sizes of Ag\u2010NPs, ZnO, and Ag\u2010NPs\/ZnO on PVP fibers were 63, 30, and 44\u2009nm, respectively. The antimicrobial activity against <jats:italic>Klebsiella pneumoniae<\/jats:italic> showed the growth inhibition zones in the bacteria plates of 23.3, 54, and 60.6\u2009mm for the samples of Ag\u2010NPs\/PVP, ZnO\/PVP, and Ag\u2010NPs\/ZnO\/PVP, respectively. The antimicrobial efficiency increased by forming nanocomposites of both ZnO nanoparticles and Ag nanoparticles inside the film.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Novel antimicrobial submicron electrospun membranes based on polyvinylpyrrolidone doped with silver, zinc oxide nanoparticles were successfully fabricated.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The fabricated samples showed bactericidal activity against <jats:italic>K. pneumoniae<\/jats:italic><\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24189","type":"journal-article","created":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T08:41:30Z","timestamp":1657010490000},"page":"3347-3355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Preparation and characterization of bioactive polyvinylpyrrolidone film via electrospinning technique"],"prefix":"10.1002","volume":"85","author":[{"given":"Mahmoud","family":"Abdelhafiz","sequence":"first","affiliation":[{"name":"Military Technical College, Kobry Elkobbah  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5194-3744","authenticated-orcid":false,"given":"Ahmed S. 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