{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:45:05Z","timestamp":1790840705990,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T00:00:00Z","timestamp":1658707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30900301"],"award-info":[{"award-number":["30900301"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY21H060001"],"award-info":[{"award-number":["LY21H060001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Zinc (Zn), an essential trace element, can stimulate bone formation and inhibit osteoclastic bone resorption, which controls the growth and maintenance of bone. However, the effect of Zn supplementation on tricalcium phosphate (TCP) wear particles\u2010induced osteolysis remains unknown. Here, we doped Zn into TCP particles (ZnTCP), and explore the protective effects of Zn on TCP particles\u2010induced osteolysis in vivo. TCP particles and ZnTCP particles were embedded under the periosteum around the middle suture of the mouse calvaria. After 2\u2009weeks, blood, the periosteal tissue, and the calvaria were collected to determine serum levels of Zn and osteocalcin, pro\u2010inflammatory cytokines, bone biochemical markers, osteoclastogenesis and bone resorption area, and to explain its mechanism. Data revealed that Zn significantly prevented TCP particles\u2010induced osteoclastogenesis and bone loss, and increased bone turnover. The Zn supplement remarkably suppressed the release of pro\u2010inflammatory cytokines including tumor necrosis factor (TNF)\u2010\u03b1, interleukin (IL)\u20101\u03b2, and IL\u20106. Immunoblotting demonstrated that Zn alleviated expression levels of ER stress\u2010related proteins such as glucose\u2010regulated protein 78 (GRP78), PKR\u2010like ER kinase (PERK), phospho\u2010PERK (p\u2010PERK), eukaryotic initiation factor 2\u03b1 (eIF2\u03b1), phospho\u2010eIF2\u03b1 (p\u2010eIF2\u03b1), activating transcription factor 4 (ATF4), inositol\u2010requiring enzyme 1\u03b1 (IRE1\u2010\u03b1) and transcription factor X\u2010box binding protein spliced (XBP1s), leading to decreasing the ratios of p\u2010PERK\/PERK and p\u2010eIF2\u03b1\/eIF2\u03b1. Taken together, Zn supplementation strongly prevents TCP particles\u2010induced periprosthetic osteolysis via inhibition of the ER stress pathway, and it may be a novel therapeutic approach for the treatment of aseptic prosthesis loosening.<\/jats:p>","DOI":"10.1002\/jemt.24213","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T08:22:39Z","timestamp":1658737359000},"page":"3608-3617","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Protective effect of zinc supplementation on tricalcium phosphate particles\u2010induced inflammatory osteolysis in mice"],"prefix":"10.1002","volume":"85","author":[{"given":"Pei","family":"Yang","sequence":"first","affiliation":[{"name":"College of Medicine Shaoxing University  Shaoxing People's Republic of 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