{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:43:17Z","timestamp":1760643797896,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T00:00:00Z","timestamp":1659484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The relationship between cartilage growth \u2013 mineralization patterns were studied in adult Rajidae with X\u2010ray morphology\/morphometry, undecalcified resin\u2010embedded, heat\u2010deproteinated histology and scanning electron microscopy. Morphometry of the wing\u2010fins, nine central rays of the youngest and oldest specimens documented a significant decrement of radials mean length between inner, middle and outer zones, but without a regular progression along the ray. This suggests that single radial length growth is regulated in such a way to align inter\u2010radial joints parallel to the wing metapterygia curvature. Trans\u2010illumination and heat\u2010deproteination techniques showed polygonal and cylindrical morphotypes of tesserae, whose aligned pattern ranged from mono\u2010columnar, bi\u2010columnar, and multi\u2010columnar up to the crustal\u2010like layout. Histology of tessellated cartilage allowed to identify of zones of the incoming mineral deposition characterized by enhanced duplication rate of chondrocytes with the formation of isogenic groups, whose morphology and topography suggested a relationship with the impending formation of the radials calcified column. The morphotype and layout of radial tesserae were related to mechanical demands (stiffening) and the size\/mass of the radial cartilage body. The cartilage calcification pattern of the batoids model shares several morphological features with tetrapods' endochondral ossification, that is, (chondrocytes' high duplication rate, alignment in rows, increased volume of chondrocyte lacunae), but without the typical geometry of the metaphyseal growth plates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p>1. The wing\u2010fins system consists of stiff radials, mobile inter\u2010radial joints and a flat inter\u2010radial membrane adapted to the mechanical demand of wing wave movement.<\/jats:p><jats:p>2. Growth occurs by forming a mixed calcified\u2010uncalcified cartilage texture, developing intrinsic tensional stresses documented by morphoanatomical data.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24217","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T03:34:50Z","timestamp":1659497690000},"page":"3642-3652","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The combined cartilage growth \u2013 calcification patterns in the wing\u2010fins of Rajidae (Chondrichthyes): A divergent model from endochondral ossification of tetrapods"],"prefix":"10.1002","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3344-2388","authenticated-orcid":false,"given":"Ugo E.","family":"Pazzaglia","sequence":"first","affiliation":[{"name":"DSMC University of Brescia  Brescia Italy"},{"name":"DMC University of Insubria  Varese Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcella","family":"Reguzzoni","sequence":"additional","affiliation":[{"name":"DMC University of Insubria  Varese Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renata","family":"Manconi","sequence":"additional","affiliation":[{"name":"DVM (Zoology Lab) University of Sassari  Sassari Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piero Antonio","family":"Zecca","sequence":"additional","affiliation":[{"name":"DMC University of Insubria  Varese Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Zarattini","sequence":"additional","affiliation":[{"name":"DSMC University of Brescia  Brescia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Campagnolo","sequence":"additional","affiliation":[{"name":"DBSV University of Insubria  Varese Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6322-1845","authenticated-orcid":false,"given":"Mario","family":"Raspanti","sequence":"additional","affiliation":[{"name":"DMC University of Insubria  Varese Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijnurstu.2009.10.001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.068981"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-197107000-00010"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.10088"},{"issue":"1","key":"e_1_2_9_6_1","first-page":"15","article-title":"Interrelationship of living elasmobranchs","volume":"53","author":"Compagno L. 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