{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:57:14Z","timestamp":1789545434460,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T00:00:00Z","timestamp":1666310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006280","name":"Ministerio de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["2004\u201002580\u2010C03\u201003\/BOS"],"award-info":[{"award-number":["2004\u201002580\u2010C03\u201003\/BOS"]}],"id":[{"id":"10.13039\/501100006280","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Hypoderma<\/jats:italic> spp. larvae were observed subcutaneously in the dorsal and lumbar regions of red deer (<jats:italic>Cervus elaphus<\/jats:italic>) hunted in the province of Le\u00f3n (northwestern Spain) causing a myiasis. They were removed and initially classified by their size, shape, color, and location under the skin into the three larval stages that parasitize these animals. The morphological characteristics of the first and second\u2010instar are described and from the features of the third\u2010instar the species was identified as <jats:italic>Hypoderma actaeon<\/jats:italic>. To accurately identify this species, five isolates of genomic DNA from the third\u2010instar, two from the second\u2010instar and two from first\u2010instar of <jats:italic>H. actaeon<\/jats:italic> were analyzed by PCR analysis of the COI region of mt\u2010DNA. The results confirmed that the examined samples exactly matched with <jats:italic>H. actaeon.<\/jats:italic> This study has shown the morphological identification of the three larval stages of <jats:italic>H. actaeon<\/jats:italic> and, for the first time, the first and second\u2010instar larvae have been molecularly characterized. Finally, identification of only <jats:italic>H. actaeon<\/jats:italic> suggests that this species is the only affecting red deer in the Iberian Peninsula.<\/jats:p>","DOI":"10.1002\/jemt.24247","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T05:50:27Z","timestamp":1666331427000},"page":"3-11","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification of <i>Hypoderma actaeon<\/i> (Diptera: Oestridae) in red deer (<i>Cervus elaphus<\/i>) from northern Spain: Microscopy study and molecular analysis"],"prefix":"10.1002","volume":"86","author":[{"given":"Sara","family":"Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Department of Animal Health, Parasitology and Parasitic Diseases, Faculty of Veterinary Science University of Le\u00f3n  Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3710-2847","authenticated-orcid":false,"given":"Maria Luisa","family":"Del Rio","sequence":"additional","affiliation":[{"name":"Department of Animal Health, Section of Immunobiology, Faculty of Veterinary Science University of Le\u00f3n  Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1113-9978","authenticated-orcid":false,"given":"Maria Natividad","family":"Diez","sequence":"additional","affiliation":[{"name":"Department of Animal Health, Parasitology and Parasitic Diseases, Faculty of Veterinary Science University of Le\u00f3n  Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0901-3997","authenticated-orcid":false,"given":"Maria del Rosario","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"Department of Animal Health, Parasitology and Parasitic Diseases, Faculty of Veterinary Science University of Le\u00f3n  Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6631-3817","authenticated-orcid":false,"given":"Angelica","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Science, Faculty of Sciences University of Burgos  Burgos Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2017.55.6.653"},{"key":"e_1_2_10_3_1","first-page":"291","volume-title":"Monographie der Oestriden","author":"Brauer F. M.","year":"1863"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470377000.ch3"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1079\/9780851996844.0098"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2915.1998.00098.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2915.2001.00287.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-46.3.934"},{"key":"e_1_2_10_9_1","volume-title":"IV Reuni\u00f3n Anual de la Asociaci\u00f3n de Parasit\u00f3logos Espa\u00f1oles","author":"Gil\u2010Collado J.","year":"1984"},{"key":"e_1_2_10_10_1","volume-title":"Molecular cloning: A laboratory manual","author":"Green M. R.","year":"2012"},{"key":"e_1_2_10_11_1","first-page":"93","article-title":"Par\u00e1sitos del ciervo (Cervus elaphus) en C\u00f3rdoba. I. Primera relaci\u00f3n","volume":"40","author":"Hern\u00e1ndez S.","year":"1980","journal-title":"Revista Ib\u00e9rica de Parasitolog\u00eda"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.1990.tb00445.x"},{"key":"e_1_2_10_13_1","first-page":"419","article-title":"Deer hypodermosis","volume":"39","author":"Mart\u00ednez\u2010Moreno F. J.","year":"1997","journal-title":"Parassitologia"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt376"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2915.2003.00446.x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2915.2003.00442.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.2010.00892.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/mve.12205"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-31.4.486"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-37.2.342"},{"key":"e_1_2_10_21_1","first-page":"85","article-title":"On the incidence of larvae of Hypodermatidae in the games and wild rodents of Hungary","volume":"9","author":"Sugar L.","year":"1976","journal-title":"Parasitologia Hungarica"},{"key":"e_1_2_10_22_1","unstructured":"Teskey H. J.(1981).Morphology and terminology\u2010larvae. In J. F. McAlpine B. V. Peterson G. E. Shewell H. J. Teskey J. R. Vockeroth & D. M. Wood (Coords.) Manual of neartic diptera(Vol. 1 pp. 65\u201388). Agriculture Canada Research Branch. Monograph 27."},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.06.033"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS15105J"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(01)00414-5"},{"key":"e_1_2_10_26_1","first-page":"217","volume-title":"Myasis in man and animals in the old world","author":"Zumpt F.","year":"1965"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24247","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jemt.24247","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24247","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:12:38Z","timestamp":1760631158000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.24247"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,21]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1002\/jemt.24247"],"URL":"https:\/\/doi.org\/10.1002\/jemt.24247","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,21]]},"assertion":[{"value":"2022-06-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}