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Resistance has been reported for all available anticoccidial drugs. Recently, green synthesis of nanoparticles is considered a new therapeutic tool against this parasitic disease. The present work aimed to study the effect of biosynthesized nanoselenium from <jats:italic>Azadirachta indica<\/jats:italic> leaf extracts (BNS) against <jats:italic>Eimeria papillata<\/jats:italic>\u2010induced infection in mice. The phytochemical analysis of leaf extracts contained 33 phytochemical components. The BNS was spherical with \u205368.12\u2009nm in diameter and an absorption peak at 308\u2009nm via UV\u2010spectra. The data showed that mice infected with <jats:italic>E. papillata<\/jats:italic> revealed the highest oocyst output on the 5th\u2010day post\u2010infection (p.i.). Infection also induced injury and inflammation of the mice jejunum. Treatment with BNS resulted in a 97.21% suppression for the oocyst output. The treated groups with BNS showed enhancement in feed intake as compared to the infected group. Histological examinations showed a significant reduction in the intracellular developmental <jats:italic>Eimeria<\/jats:italic> stages in the jejunal tissues of infected\u2010treated mice of about 24.86\u2009\u00b1\u20092.38 stages\/10 villous crypt units. Moreover, there was a significant change in the morphometry for <jats:italic>Eimeria<\/jats:italic> stages after the treatment with BNS. Infection induced a disturbance in the level of carbohydrates and protein contents in the infected mice which enhanced after treatment with BNS. In addition, BNS counteracted the <jats:italic>E. papillata<\/jats:italic>\u2010induced loss of the total antioxidant capacity. Collectively, BNS is considered a promising anticoccidial and antioxidant effector and could be used for the treatment of coccidiosis.<\/jats:p>","DOI":"10.1002\/jemt.24331","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T07:10:47Z","timestamp":1682061047000},"page":"714-724","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of biosynthesized nanoselenium using <i>Azadirachta indica<\/i> (Meliaceae) leaf extracts against <i>Eimeria papillata<\/i> infection"],"prefix":"10.1002","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9263-6871","authenticated-orcid":false,"given":"Rewaida","family":"Abdel\u2010Gaber","sequence":"first","affiliation":[{"name":"Department of Zoology, College of Sciences King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maysar Abu","family":"Hawsah","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Sciences King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esam M.","family":"Al\u2010Shaebi","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Sciences King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahani","family":"Al\u2010Otaibi","sequence":"additional","affiliation":[{"name":"Department of Science and Technology Al\u2010Nairiyah University College, University of Hafr Al\u2010Batin  Hafr Al\u2010Batin Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felwa A.","family":"Thagfan","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science Princess Nourah Bint Abdulrahman University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saleh","family":"Al\u2010Quraishy","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Sciences King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1869-5800","authenticated-orcid":false,"given":"Mohamed A.","family":"Dkhil","sequence":"additional","affiliation":[{"name":"Department of Zoology and Entomology, Faculty of Science Helwan University  Cairo Egypt"},{"name":"Applied Science Research Center Applied Science Private University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S004393391100033X"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1139899"},{"key":"e_1_2_11_4_1","volume-title":"Arbeitsmethoden der makroskopischen und mikroskopischen anatomic. 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