{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T20:32:11Z","timestamp":1780605131979,"version":"3.54.1"},"reference-count":111,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T00:00:00Z","timestamp":1682726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Fabrication, characterization and evaluation of the larvicidal potential of novel silk protein (sericin)\u2010based silver nanoparticles (Se\u2010AgNPs) were the prime motives of the designed study. Furthermore, investigation of the sericin as natural reducing or stabilizing agent was another objective behind this study. Se\u2010AgNPs were synthesized using sonication and heat. Fabricated Se\u2010AgNPs were characterized using particle size analyzer, UV spectrophotometry, FTIR and SEM which confirmed the fabrication of the Se\u2010AgNPs. Size of sonication\u2010mediated Se\u2010AgNPs was smaller (7.49\u2009nm) than heat\u2010assisted Se\u2010AgNPs (53.6\u2009nm). Being smallest in size, sonication\u2010assisted Se\u2010AgNPs revealed the significantly highest (<jats:italic>F<\/jats:italic><jats:sub>4,10<\/jats:sub>\u2009=\u200939.20, <jats:italic>p<\/jats:italic>\u2009=\u2009.00) larvicidal activity against fourth instar lab and field larvae (<jats:italic>F<\/jats:italic><jats:sub>4,10<\/jats:sub>\u2009=\u20091864, <jats:italic>p<\/jats:italic>\u2009=\u2009.00) of dengue vector (<jats:italic>Aedes aegypti<\/jats:italic>) followed by heat\u2010assisted Se\u2010AgNPs and positive control (temephos). Non\u2010significant larvicidal activity was showed by silver (without sericin) which made the temperature stability of silver, debatable. Furthermore, findings of biochemical assays (glutathione\u2010S transferase, esterase, and acetylcholinesterase) showed the levels of resistance in field strain larvae. Aforementioned findings of the study suggests the sonication as the best method for synthesis of Se\u2010AgNPs while the larvicidal activity is inversely proportional to the size of Se\u2010AgNPs, i.e., smallest the size, highest the larvicidal activity. Conclusively, status of the sericin as a natural reducing\/stabilizing agent has been endorsed by the findings of this study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Incorporation of biocompatible and inexpensive sericin as a capping\/reducing agent for synthesis of Se\u2010AgNPs.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>A novel sonication method was used for the fabrication of Se\u2010AgNPs which were thoroughly characterized by particle size analyzer, UV\u2013visible spectrophotometry, SEM and FTIR.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Analysis of enzymatic (GSTs, ESTs) levels in field and lab strains of <jats:italic>Aedes aegypti<\/jats:italic> larvae for evaluation of insecticides resistance.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24333","type":"journal-article","created":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T08:09:40Z","timestamp":1682755780000},"page":"1363-1377","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Sonication and heat\u2010mediated synthesis, characterization and larvicidal activity of sericin\u2010based silver nanoparticles against dengue vector (<i>Aedes aegypti<\/i>)"],"prefix":"10.1002","volume":"86","author":[{"given":"Muhammad","family":"Summer","sequence":"first","affiliation":[{"name":"Laboratory of Applied Entomology and Medical Toxicology, Department of Zoology Government College University Lahore  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hafiz Muhammad","family":"Tahir","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Entomology and Medical Toxicology, Department of Zoology Government College University Lahore  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaukat","family":"Ali","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Entomology and Medical Toxicology, Department of Zoology Government College University Lahore  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2021.11.013"},{"key":"e_1_2_11_3_1","first-page":"1","article-title":"Dynamics of insecticide resistance and effect of synergists piperonyl butoxide (PBO), SSS\u2010tributylphosphorotrithioate (DEF) and ethacrynic acid (ETAA or EA) on permethrin, deltamethrin and dichlorodiphenyltrichloroethane (DDT) resistance in two Anopheles gambiae s.l. populations from southern Benin, West Africa","volume":"6","author":"A\u00efzoun N.","year":"2014","journal-title":"Journal of Parasitology and Vector Biology"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjx196"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1049\/iet-nbt.2015.0123"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10876-019-01542-7"},{"issue":"1","key":"e_1_2_11_7_1","first-page":"1","article-title":"Green synthesis of silk sericin\u2010capped silver nanoparticles and their potent anti\u2010bacterial activity","volume":"9","author":"Aramwit P.","year":"2014","journal-title":"Nanoscale Research Letters"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-016-9897-y"},{"issue":"8","key":"e_1_2_11_9_1","first-page":"198","article-title":"Effectiveness of inflammatory cytokines induced by sericin compared to sericin in combination with silver sulfadiazine cream on wound healing","volume":"21","author":"Aramwit P.","year":"2009","journal-title":"Wounds: A Compendium of Clinical Research and Practice"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-017-0898-0"},{"issue":"1","key":"e_1_2_11_11_1","first-page":"42","article-title":"Green synthesis, characterization of silver sulfide nanoparticles and antibacterial activity evaluation","volume":"6","author":"Awwad A. M.","year":"2020","journal-title":"Chemistry Internaational"},{"key":"e_1_2_11_12_1","unstructured":"Baptista P. A. R.(2018).Nanotechnology approaches for topical delivery of minoxidil (Doctoral dissertation Universidade de Coimbra)."},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.11.101"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-018-1850-4"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-017-5395-0"},{"key":"e_1_2_11_16_1","first-page":"168","article-title":"Use of sericin of Bombyx mori in the synthesis of silver nanoparticles, their characterization and application","volume":"36","author":"Bhat P. N.","year":"2011","journal-title":"Indian Journal of Fibre and Textile Research"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22050721"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/fes3.108"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10311-016-0600-4"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/107555302760253577"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45261-5"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-15-9456-4_6"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-013-9667-6"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2020.110822"},{"key":"e_1_2_11_25_1","first-page":"1","article-title":"A critical review on application of silk sericin and its mechanical properties in various industries","volume":"9","author":"Fatahian R.","year":"2021","journal-title":"Journal of Research and Applications in Mechanical Engineering"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.15282\/ijets.9.1.2022.1001"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2016.1241793"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvec.12355"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2017.11.004"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00289-019-02820-0"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10876-019-01596-7"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5837"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12440"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2017.06.015"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2004.03.018"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2016.88"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1900"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2022.11.019"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/21870764.2018.1439606"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2019.101174"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986709788612729"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10876-018-1441-z"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2017.05.011"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-46835-8_15"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2011.01.003"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-017-5711-8"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-020-08444-6"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.8b00558"},{"issue":"4","key":"e_1_2_11_49_1","first-page":"191","article-title":"The role of biopesticides in sustainably feeding the nine billion global populations","volume":"29","author":"Kumar S.","year":"2013","journal-title":"Journal of Biofertilizers and Biopesticides"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2008.08.002"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.7b00549"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-13296-5_9"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.15406\/jnmr.2017.06.00158"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr068445e"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2015.04.002"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-8130(03)00069-2"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20040865"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3472-1"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19102875"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079928"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/6917938"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.24273"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/205183"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-815829-6.00013-9"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjab137"},{"issue":"1","key":"e_1_2_11_66_1","first-page":"1","article-title":"Dynamics of insecticide resistance and effect of synergists piperonyl butoxide (PBO), SSS\u2010tributylphosphorotrithioate (DEF) and ethacrynic acid (ETAA or EA) on permethrin, deltamethrin and dichlorodiphenyltrichloroethane (DDT) resistance in two Anopheles gambiae sl populations from southern Benin, West Africa","volume":"6","author":"Nazaire A.","year":"2014","journal-title":"Journal of Parasitology and Vector Biology"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145835"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-020-02971-5"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.btre.2018.e00297"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.17221\/31\/2016-PPS"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2010.09.005"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10876-019-01606-8"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2017.11.028"},{"issue":"2","key":"e_1_2_11_74_1","first-page":"528","article-title":"Nanotechnology for insect pest control","volume":"3","author":"Ragaei M.","year":"2014","journal-title":"International Journal of Science, Environment and Technology"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00427"},{"issue":"6","key":"e_1_2_11_76_1","first-page":"1031","article-title":"Application of sericin in textile industry","volume":"12","author":"Rajalakshmi M.","year":"2020","journal-title":"Journal of Interdisciplinary Cycle Research"},{"key":"e_1_2_11_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2020.104566"},{"key":"e_1_2_11_78_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.200540"},{"key":"e_1_2_11_79_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1020-49891998001000004"},{"key":"e_1_2_11_80_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-017-1744-9"},{"key":"e_1_2_11_81_1","doi-asserted-by":"publisher","DOI":"10.57182\/jbiopestic.9.1.63-72"},{"key":"e_1_2_11_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jallcom.2018.07.202"},{"key":"e_1_2_11_83_1","first-page":"1","article-title":"Antibacterial, cytotoxicity and larvicidal activity of green synthesized selenium nanoparticles using Penicilliumcorylophilum","author":"Salem S. S.","year":"2020","journal-title":"Journal of Cluster Science"},{"key":"e_1_2_11_84_1","doi-asserted-by":"publisher","DOI":"10.3923\/jas.2010.3132.3136"},{"key":"e_1_2_11_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2019.04.046"},{"key":"e_1_2_11_86_1","doi-asserted-by":"publisher","DOI":"10.1080\/01480545.2018.1525393"},{"key":"e_1_2_11_87_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612825666190708185506"},{"key":"e_1_2_11_88_1","article-title":"Morphological and physiological changes induced by Achyranthes aspera\u2010mediated silver nanocomposites in Aedes aegypti larvae","volume":"21","author":"Sharma A.","year":"2022","journal-title":"Frontiers in Physiology"},{"key":"e_1_2_11_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eurpolymj.2021.110960"},{"key":"e_1_2_11_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2016.02.006"},{"key":"e_1_2_11_91_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10924-016-0822-3"},{"key":"e_1_2_11_92_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2017.12.015"},{"key":"e_1_2_11_93_1","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.202200632"},{"key":"e_1_2_11_94_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4095(20020605)14:11<833::AID-ADMA833>3.0.CO;2-K"},{"key":"e_1_2_11_95_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-3138-9"},{"key":"e_1_2_11_96_1","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.201900567"},{"key":"e_1_2_11_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2019.03.111"},{"key":"e_1_2_11_98_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9EM00586B"},{"key":"e_1_2_11_99_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13346-016-0322-y"},{"key":"e_1_2_11_100_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aspen.2010.03.003"},{"key":"e_1_2_11_101_1","unstructured":"WHO. (1996).Guidelines for laboratory and field testing of mosquito larvicides (No. WHO\/CDS\/WHOPES\/GCDPP\/2005.13). World Health Organization."},{"key":"e_1_2_11_102_1","unstructured":"WHO. (2015).Dengue and severe dengue. Fact sheet N\u00b0117 Geneva World Health Organization."},{"key":"e_1_2_11_103_1","unstructured":"WHO. (2019a).Lymphatic filariasis. Fact sheet N\u00b0102. World Health Organization Geneva."},{"key":"e_1_2_11_104_1","unstructured":"WHO. (2019b)Guidance on management of household pesticides."},{"key":"e_1_2_11_105_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.25220"},{"key":"e_1_2_11_106_1","doi-asserted-by":"publisher","DOI":"10.1166\/sam.2018.3258"},{"key":"e_1_2_11_107_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aas8815"},{"key":"e_1_2_11_108_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-1792-8"},{"key":"e_1_2_11_109_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fuel.2014.05.058"},{"key":"e_1_2_11_110_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2nr31281f"},{"key":"e_1_2_11_111_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00114"},{"key":"e_1_2_11_112_1","doi-asserted-by":"publisher","DOI":"10.3390\/su12187523"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24333","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:47:16Z","timestamp":1760626036000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.24333"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,29]]},"references-count":111,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1002\/jemt.24333"],"URL":"https:\/\/doi.org\/10.1002\/jemt.24333","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,29]]},"assertion":[{"value":"2023-01-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}