{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T07:36:15Z","timestamp":1785569775389,"version":"3.56.0"},"reference-count":69,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,5,28]],"date-time":"2023-05-28T00:00:00Z","timestamp":1685232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The current study aims to utilize the bacteria <jats:italic>Paraclostridium benzoelyticum<\/jats:italic> strain 5610 to synthesize bio\u2010genic silver nanoparticles (AgNPs). Biogenic AgNPs were thoroughly examined using various characterization techniques such as UV\u2010spectroscopy, XRD, FTIR, SEM, and EDX. Synthesis of AgNPs was confirmed by UV\u2013vis analysis resulting in absorption peak at 448.31\u2009nm wavelength. The SEM analysis indicated the morphological characteristics and size of AgNPs which was 25.29\u2009nm. The face centered cubic (FCC) crystallographic structure was confirmed by XRD. Furthermore, FTIR study affirmed the capping of AgNPs by different compounds found in biomass of the <jats:italic>Paraclostridium benzoelyticum<\/jats:italic> strain 5610. Later, EDX was used to determine the elemental composition with respective concentration and distribution. Additionally, in the current study the antibacterial, anti\u2010inflammatory, antioxidant, anti\u2010aging, and anti\u2010cancer ability of AgNPs was assessed. The antibacterial activity of AgNPs was tested against four distinct sinusitis pathogens: <jats:italic>Haemophilus in\u2010fluenza, Streptococcus pyogenes, Moraxella catarrhalis<\/jats:italic> and <jats:italic>Streptococcus pneumonia.<\/jats:italic> AgNPs shows significant inhibition zone against <jats:italic>Streptococcus pyogenes<\/jats:italic> 16.64\u2009\u00b1\u20090.35 followed by 14.32\u2009\u00b1\u2009071 for <jats:italic>Moraxella catarrhalis.<\/jats:italic> Similarly, the antioxidant potential was found maximum (68.37\u2009\u00b1\u20090.55%) at 400\u2009\u03bcg\/mL and decrease (5.48\u2009\u00b1\u20090.65%) at 25\u2009\u03bcg\/mL, hence the significant antioxidant ability was observed. Furthermore, anti\u2010inflammatory activity of AgNPs shows the strongest inhibitory action (42.68\u2009\u00b1\u20090.62%) for 15\u2010LOX with lowest inhibition activity for COX\u20102 (13.16\u2009\u00b1\u20090.46%). AgNPs have been shown to exhibit significant inhibitory actions against the enzyme elastases AGEs (66.25\u2009\u00b1\u20090.49%), which are followed by AGEs of visperlysine (63.27\u2009\u00b1\u20090.69%). Furthermore, the AgNPs show high toxicity against HepG2 cell line which shows 53.543% reduction in the cell viability after 24\u2009h of treatment. The anti\u2010inflammatory activity demonstrated a potent inhibitory effect of the bio\u2010inspired AgNPs. Overall, the biogenic AgNPs have the ability to be served for the treatments of anti\u2010aging and also due to their anti\u2010cancer, antioxidant abilities NPs may be a useful therapy choice for a variety of disorders including cancer, bacterial infections and other inflammatory diseases. Moreover, further studies are required in the future to evaluate their in vivo biomedical applications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Biogenic synthesis of AgNPs using Paraclostridium benzoelyticum Strain for the first time.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>FTIR analysis confirmed capping of potent biomolecules which are of great use in applied field especially Nanomedicines.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Notable antimicrobial activity against sinusitis bacteria and cytotoxic potential of synthesized AgNPs on in vitro basis produce a new idea shifting us to treat cancerous cell lines.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24362","type":"journal-article","created":{"date-parts":[[2023,5,28]],"date-time":"2023-05-28T08:25:47Z","timestamp":1685262347000},"page":"846-861","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Biofabrication of silver nanoparticles employing biomolecules of <i>Paraclostridium benzoelyticum<\/i> strain: Its characterization and their in\u2010vitro antibacterial, anti\u2010aging, anti\u2010cancer and other biomedical applications"],"prefix":"10.1002","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5474-2622","authenticated-orcid":false,"given":"Shah","family":"Faisal","sequence":"first","affiliation":[{"name":"Institute of Biotechnology and Microbiology Bacha Khan University  Charsadda Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riaz","family":"Ullah","sequence":"additional","affiliation":[{"name":"Medicinal Aromatic and Poisonous Plants Research Center College of Pharmacy, King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Alotaibi","sequence":"additional","affiliation":[{"name":"Department of Basic Science College of Medicine, Princess Nourah bint Abdulrahman University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sania","family":"Zafar","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Biotechnology Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Rizwan","sequence":"additional","affiliation":[{"name":"Center for Biotechnology and Microbiology University of Swat  Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1261-7481","authenticated-orcid":false,"given":"Muhammad Hamza","family":"Tariq","sequence":"additional","affiliation":[{"name":"Department of Chemistry National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,28]]},"reference":[{"issue":"2","key":"e_1_2_10_2_1","first-page":"763","article-title":"Green synthesis of silver nanoparticles using Bidens frondosa extract and their tyrosinase activity","volume":"16","author":"Abbas Q.","year":"2017","journal-title":"Iranian Journal of Pharmaceutical Research: IJPR"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S239513"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2015.02.007"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano11082142"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2017.07.003"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-006-9063-1"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13204-015-0473-z"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cplett.2022.139493"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1RA08098A"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00016-10"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.12.989"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym13111680"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3906\/biy-1801-53"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacsau.2c00366"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5994033"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-838220100002000028"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8RA10483B"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.15171\/bi.2016.29"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1049\/nbt2.12070"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-014-0210-4"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1049\/iet-nbt.2017.0089"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.5b00370"},{"issue":"3","key":"e_1_2_10_24_1","first-page":"104","article-title":"Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity","volume":"2","author":"Guzm\u00e1n M. G.","year":"2009","journal-title":"International Journal of Chemical and Biomolecular Engineering"},{"issue":"3","key":"e_1_2_10_25_1","first-page":"12","article-title":"Antimicrobial activity and biomedical application of Sambucus wighfiana phenolic extract against gram positive and gram\u2010negative strains of bacteria","volume":"9","author":"Hassan N.","year":"2020","journal-title":"Journal of Biomedical Science"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2007.01.018"},{"issue":"6","key":"e_1_2_10_27_1","first-page":"385","article-title":"Synthesis of silver nanoparticles: Chemical, physical and biological methods","volume":"9","author":"Iravani S.","year":"2014","journal-title":"Research in Pharmaceutical Sciences"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmrt.2021.08.048"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmrt.2021.08.048"},{"issue":"3","key":"e_1_2_10_30_1","first-page":"991","article-title":"Green synthesis of silver nanoparticles from extract of Padina tetrastromatica leaf","volume":"7","author":"Jegadeeswaran P.","year":"2012","journal-title":"Digest Journal of Nanomaterials and Biostructures"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3109\/21691401.2015.1068792"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.833154"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2017.05.011"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/aoc.6213"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2015.12.011"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232112939"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01153"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1088\/1742-6596\/146\/1\/012025"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2014.10.043"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2019.110350"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.23893\/1307-2080.APS.05708"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1049\/nbt2.12036"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2021.102222"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e04164"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26227031"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym12061418"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/2228-5326-2-32"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2012.05253.x"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S265003"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.08.179"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-3155-2-3"},{"key":"e_1_2_10_52_1","first-page":"238","article-title":"Evaluation and comparison the antibacterial activity of silver nano particles (AgNPs) and silver nitrate (AgNO3) on some pathogenic bacteria","volume":"9","author":"Salman H. D.","year":"2017","journal-title":"Journal of Global Pharma Technology"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.2147\/NSA.S133415"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2020.110889"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmt.2016.09.009"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.3109\/21691401.2015.1052472"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S72313"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S72313"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.3109\/21691401.2015.1064937"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2016.02.006"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/234741"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00449-008-0224-6"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2221-1691(13)60006-4"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/jccs.201700067"},{"issue":"4","key":"e_1_2_10_65_1","first-page":"1127","article-title":"Green synthesis of silver nanoparticles by Bacillusmethylotrophicus, and their antimicrobial activity","volume":"44","author":"Wang C.","year":"2016","journal-title":"Artif Cells Nanomed Biotechnol."},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfa.2005.08.019"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.45413"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.17719\/jisr.2018.2730"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma12244180"},{"key":"e_1_2_10_70_1","first-page":"1","article-title":"Silver nanoparticles potentiates cytotoxicity and apoptotic potential of camptothecin in human cervical cancer cells","volume":"2018","author":"Yuan Y. G.","year":"2018","journal-title":"Oxidative Medicine and Cellular Longevity"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24362","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:16:22Z","timestamp":1760631382000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.24362"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,28]]},"references-count":69,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1002\/jemt.24362"],"URL":"https:\/\/doi.org\/10.1002\/jemt.24362","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,28]]},"assertion":[{"value":"2023-03-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}