{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T19:40:28Z","timestamp":1768678828075,"version":"3.49.0"},"reference-count":59,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:00:00Z","timestamp":1687910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Down syndrome (DS) is a genetically based disease caused by triplication of chromosome 21. DS is characterized by multi\u2010systemic premature aging associated with deficit in motor coordination, balance, and postural control. Using a morphological, morphometrical, and immunocytochemical ultrastructural approach, this study investigated in vastus lateralis muscle of Ts65Dn mouse, a murine model of DS, the effect of an adapted physical training on the extracellular matrix (ECM) characteristics and whether the forecasted exercise\u2010induced ECM remodeling impacts on sarcomere organization. Morphometry demonstrated thicker basement membrane and larger collagen bundles with larger interfibrillar spacing as well as irregularly arrayed myofibrils and lower telethonin density on Z\u2010lines in trisomic versus euploid sedentary mice. In agreement with the multi\u2010systemic premature aging described in DS, these ECM alterations were similar to those previously observed in skeletal muscle of aged mice. Adapted physical training induced remodeling of ECM in both trisomic and euploid mice, that is, enlargement of the collagen bundles associated with hypertrophy of collagen fibrils and reduction of the interfibrillar spacing. A re\u2010alignment of the myofibrils and a higher telethonin density on Z\u2010line was found in trisomic mice. Altogether, our findings suggest that physical training is an effective tool in limiting\/counteracting the trisomy\u2010associated musculoskeletal structural anomalies. The current findings constitute a solid experimental background for further study investigating the possible positive effect of physical training on skeletal muscle performance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Vastus lateralis muscle of trisomic mice shows aging\u2010like alterations of extracellular matrix.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Training promotes extracellular matrix remodeling.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Training may be an effective tool to counteract trisomy\u2010associated alterations of skeletal 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