{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:27:42Z","timestamp":1790166462482,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T00:00:00Z","timestamp":1687996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700936"],"award-info":[{"award-number":["81700936"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LTGY23H180009"],"award-info":[{"award-number":["LTGY23H180009"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY15H180012"],"award-info":[{"award-number":["LY15H180012"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY21H060001"],"award-info":[{"award-number":["LY21H060001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY21H170002"],"award-info":[{"award-number":["LY21H170002"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>During orthodontic tooth movement (OTM), osteocytes, the most mechanosensitive cells in alveolar bone, suffer the heavy orthodontic force and initiate alveolar bone resorption on the compression side. However, the inherent mechanisms of compressive force\u2010induced osteocyte death are not fully understood. In this study, we established an OTM model on Sprague\u2013Dawley rats by inserting coil springs to investigate osteocyte damage on the compression side of alveolar bone. We then applied compressive force on the MLO\u2010Y4 osteocyte\u2010like cell line in vitro to explore whether the reactive oxygen species (ROS)\u2010mediated endoplasmic reticulum stress (ERS) pathway is involved in compressive force\u2010induced osteocyte death. We found that the orthodontic force caused apparent alveolar bone loss, osteocyte death, and elevated serum sclerostin and receptor activator of NF\u2010\u03baB ligand (RANKL) levels in rats. In vitro, compressive force inhibited cell viability but increased the LDH leakage and loss of mitochondrial membrane potential in MLO\u2010Y4 cells. Simultaneously, it activated protein kinase RNA\u2010like endoplasmic reticulum kinase (PERK), eukaryotic translation initiation factor 2 (eIF2\u03b1), and their downstream pro\u2010apoptotic ERS signaling proteins and caused significant osteocyte apoptosis, which can be blocked by ERS inhibitor salubrinal. Moreover, the compressive force elevated intracellular ROS levels, while the ROS scavenger N\u2010acetyl\u2010L\u2010cysteine (NAC) alleviated ERS and apoptosis in loaded osteocytes. These results suggest that the orthodontic compressive force induced osteocyte apoptosis via the ROS\u2010mediated ERS pathway. This study first proposes the ERS pathway as a new potential pathway for regulating the rate of OTM based on osteocyte death.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>The orthodontic force increases osteocyte death in rat alveolar bone.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The compressive force causes osteocyte apoptosis by the endoplasmic reticulum stress (ERS) pathway in vitro.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The ROS scavenger NAC blocked compressive force\u2010induced ERS and osteocyte apoptosis.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24382","type":"journal-article","created":{"date-parts":[[2023,6,29]],"date-time":"2023-06-29T07:41:10Z","timestamp":1688024470000},"page":"1529-1541","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Reactive oxygen species\u2010mediated endoplasmic reticulum stress contributes to osteocyte death induced by orthodontic compressive force"],"prefix":"10.1002","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8866-2241","authenticated-orcid":false,"given":"Ming","family":"Yan","sequence":"first","affiliation":[{"name":"School of Automation Hangzhou Dianzi University  Hangzhou People's Republic of 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