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Neotropical bats have been poorly studied, and important aspects on sperm morphology have not been elucidated. The aim of the present study was to describe and compare the sperm morphology and morphometry of <jats:italic>Molossus molossus<\/jats:italic> and <jats:italic>Molossops temminckii<\/jats:italic>. A total of 14 adults specimens were analyzed from the Colecci\u00f3n Mam\u00edferos Lillo, Universidad Nacional de Tucum\u00e1n: five <jats:italic>M. molossus<\/jats:italic> and nine <jats:italic>M. temminckii<\/jats:italic>. The epididymis were extracted and macerated in Farmer's solution, followed by a coloration with different stains. To carry out the description and morphometric analysis, microphotographs were taken under an optical, epifluorescence, and scanning electron microscope (SEM). A total of 50 sperm from each individual were measured for morphometric analysis. The length and width of the head, midpiece and tail were taken as variables. Sperm from <jats:italic>M. molossus<\/jats:italic> and <jats:italic>M. temminckii<\/jats:italic> were practically identical, both morphologically and morphometrically. In both species, a distal bulge was observed at the end of the intermediate piece in a percentage greater than 85%. The main characteristics shared between the species were: presence of acrosomal blebs in the upper half of the head of the spermatozoa; cephalic equatorial segment with filiform ornamentations; intermembrane space of head apex wedge\u2010shaped; helical middle piece and annulus at the end of middle piece. In the present study, SEM allowed us to visualize structures, such as acrosomal vesicles, that were not detected with other types of microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>The similarities in the sperm morphology between <jats:italic>M. molossus<\/jats:italic> and <jats:italic>M. temminckii<\/jats:italic> were observed with three types of microscopy: optical, epifluorescence and scanning electron, and supported by morphometric and statistical analyses.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24396","type":"journal-article","created":{"date-parts":[[2023,8,12]],"date-time":"2023-08-12T06:58:49Z","timestamp":1691823529000},"page":"1626-1634","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Sperm morphology and comparison morphometry measurements of two species of bats, <i>Molossus molossus<\/i> and <i>Molossops temminckii<\/i> (Chiroptera: Molossidae)"],"prefix":"10.1002","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-0276-0704","authenticated-orcid":false,"given":"Ana Sof\u00eda","family":"Dip","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Naturales e Instituto Miguel Lillo Universidad Nacional de Tucum\u00e1n  Tucum\u00e1n Argentina"},{"name":"CONICET NOA Sur (Centro Cient\u00edfico Tecnol\u00f3gico\u2013Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas)  Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Naturales e Instituto Miguel Lillo Universidad Nacional de Tucum\u00e1n  Tucum\u00e1n Argentina"},{"name":"Instituto de Gen\u00e9tica y Microbiolog\u00eda Fundaci\u00f3n Miguel Lillo  Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rub\u00e9n Aldo","family":"Andrada","sequence":"additional","affiliation":[{"name":"Instituto de Gen\u00e9tica y Microbiolog\u00eda Fundaci\u00f3n Miguel Lillo  Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela Beatriz","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda Animal Fundaci\u00f3n Miguel Lillo  Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Daniela","family":"Miotti","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Naturales e Instituto Miguel Lillo Universidad Nacional de Tucum\u00e1n  Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0717-95022012000400045"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.01.015"},{"key":"e_1_2_9_4_1","first-page":"262","volume-title":"Bats: Biology and behavior","author":"Altringham J. 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