{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T23:28:22Z","timestamp":1768519702846,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T00:00:00Z","timestamp":1693526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP17K10503"],"award-info":[{"award-number":["JP17K10503"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K11539"],"award-info":[{"award-number":["JP20K11539"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Midthermic machine perfusion (MMP) of post\u2010circulatory arrest donor liver grafts has the advantage of preserving the functional ultrastructure of hepatocytes in donor grafts. It was reported that oxygenation during MMP reduces portal venous resistance and increases bile production. The MMP with hemoglobin\u2010based oxygen vesicles (HbV) keeps the lower aspartate aminotransferase level (an indicator of liver injury) and maintains the functional ultrastructure of mitochondria in the hepatocytes. To evaluated differences of ultrastructural damages in donor livers between the MMP with and without HbV, porcine liver grafts after 60\u2009min of warm ischemia were perfused at 22\u00b0C for 4\u2009h with or without HbV, and a part of liver grafts were analyzed by transmission electron microscopy (TEM) and osmium\u2010maceration scanning electron microscopy (OM\u2010SEM). The remaining grafts were perfused with autologous blood at 38\u00b0C for 2\u2009h in an isolated liver reperfusion model (IRM) that mimics the inside of the body after transplantation, and then analyzed by TEM and OM\u2010SEM. Hepatocytes after MMP had small round mitochondria with rod\u2010shaped cristae and reticulovesicular rough endoplasmic reticulum (rER) in both HbV(+) and HbV(\u2212) livers. After IRM of HbV(+) livers, the well\u2010developed lamellar rER was often found in hepatocytes. Liver sinusoidal endothelial cells (LSECs) after MMP contained some large vacuolar structures containing amorphous garbage in the cytoplasm, and their size along with appearance frequency were smaller and lower, respectively, in HbV(+) livers than HbV(\u2212). Oxygenation during the MMP by using HbV suppressed the ultrastructural damages in donor livers, in particular for the LSECs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Liver sinusoidal endothelial cells after midthermic machine perfusion had large vacuolar organelles with amorphous garbage.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Oxygenation during the perfusion made them less and smaller, ultrastructurally supporting its utility.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24405","type":"journal-article","created":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T16:34:59Z","timestamp":1693586099000},"page":"1725-1732","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Machine perfusion preservation with hemoglobin based oxygen vesicles alleviate ultrastructural damages in porcine liver donated after cardiac death"],"prefix":"10.1002","volume":"86","author":[{"given":"Nur Khatijah Mohd","family":"Zin","sequence":"first","affiliation":[{"name":"Department of Cell Physiology The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1708-0348","authenticated-orcid":false,"given":"Hiroki","family":"Bochimoto","sequence":"additional","affiliation":[{"name":"Department of Cell Physiology The Jikei University School of Medicine  Tokyo Japan"},{"name":"Department of Transplantation Technology and Therapeutic Development Asahikawa Medical University  Asahikawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3015-5156","authenticated-orcid":false,"given":"Daisuke","family":"Kondoh","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy Obihiro University of Agriculture and Veterinary 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