{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T05:17:02Z","timestamp":1782278222622,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T00:00:00Z","timestamp":1698451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","award":["2022JQ\u2010987"],"award-info":[{"award-number":["2022JQ\u2010987"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Remote ischemic postconditioning (RIPostC) alleviates brain ischemic injury through several pathways, including endoplasmic reticulum (ER) stress modulation. Sarco endoplasmic reticulum Ca<jats:sup>2+<\/jats:sup>\u2010ATPase(SERCA2) which plays vital role in calcium homeostasis regulation could modulate ER stress logically. This study aimed to investigate whether RIPostC exerts its neuroprotective effect by reducing ER stress mediated by SERCA2. Male SD rats underwent transient middle cerebral artery occlusion (tMCAO) for 2\u2009h followed by reperfusion, with the RIPostC group undergoing 3\u2009cycles of bilateral femoral artery clamping and reperfusion at the beginning of reperfusion. Stroke outcome was assessed based on infarct volume and neurological function evaluation. Protein levels of SERCA2 and other ER stress markers were measured using Western blotting, immunofluorescence, and immunohistochemistry techniques. Compared to the sham group, we observed that RIPostC can effectively reduce cerebral infarct volume after I\/R (34.55%: 21.03%; <jats:italic>p<\/jats:italic>\u2009=\u2009.004) and improve neurological function deficit (9.67:12.5; <jats:italic>p<\/jats:italic>\u2009=\u2009.029). Additionally, RIPostC increased SERCA2 protein expression and decreased the protein level of glucose\u2010regulated protein 78 (GRP78), phosphorylation of eukaryotic translation initiation factor 2\u03b1 (p\u2010eIF2\u03b1) and CCAAT\/EBP homologous protein (CHOP). Furthermore, B\u2010cell lymphoma\u20102 (Bcl\u20102) expression was increased, while Bcl\u20102\u2010associated X protein (Bax) and cleaved\u2010caspase\u20103 was decreased in response to application of RIPostC. Our results suggest that RIPostC improves the prognosis of tMCAO rats, possibly by inhibiting the ER stress mediated by SERCA2, facilitating apoptosis downregulation. The significance of this study is to provide a theoretical basis for further exploring the protective mechanism of ischemic stroke by RIPostC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Our results suggest that RIPostC improves the prognosis of tMCAO rats, possibly by inhibiting the ER stress mediated by SERCA2, facilitating apoptosis downregulation, thus achieving a neuroprotective effect.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24431","type":"journal-article","created":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T08:58:10Z","timestamp":1698483490000},"page":"424-433","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Remote ischemic postconditioning alleviates cerebral ischemic injury through <scp>SERCA2<\/scp>\/endoplasmic reticulum stress\u2010mediated apoptosis"],"prefix":"10.1002","volume":"87","author":[{"given":"Wanying","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawen","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Neurology The First Affiliated Hospital of Xi'an Jiaotong University  Xi'an 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