{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T03:14:20Z","timestamp":1792206860243,"version":"4.4.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The genus <jats:italic>Achyranthes<\/jats:italic> belong to the family Amaranthaceae which constitutes an important group of herbs and shrubs with immense medicinal value. The present research work was conducted to investigate the anticancer potential of <jats:italic>Achyranthes aspera<\/jats:italic> L. leaves by focusing on the antioxidant, aniproliferative and antimitotic activities of leaf extracts. Plant extraction was carried out by soxhelt method with different solvents. Phytochemical characterization of the plants extracts using chemical methods identified the presence of cardiac glycosides, saponins, coumarins, proteins, tannins, flavonoids and triterpenes. Alkaloid was present in methanolic and ethanolic extract. High performance liquid chromatography showed presence of different concentration of myricetin, quercetin and kaempferol in different extracts with the highest concentration of myricetin (84.53\u2009\u03bcg\/mL) in n\u2010butanolic extract. The extracts were then tested for antioxidant activity using 2,2\u2010diphenylpicrylhydrazyl (DPPH) radical scavenging assay by spectrophotometric method. In DPPH radical scavenging assay, antioxidant activity of <jats:italic>A. aspera<\/jats:italic> ranged between 79.78\u2009\u00b1\u20090.034% and 58.63\u2009\u00b1\u20090.069%. Highest antioxidant activity was observed for methanolic extract and lowest for acetone. Antimitotic activity was determined by using <jats:italic>Allium cepa<\/jats:italic> assay in which microscopic investigation was carried out to observe normal and abnormal phases of mitosis. In this assay, n\u2010butanolic extract had highest antimitotic activity with minimum mitotic index at 2\u2009mg\/mL (57\u2009\u00b1\u20090.0351%). The plant extracts also caused chromosomal and mitotic aberrations which were clearly observed under 40\u00d7 and 100\u00d7 magnification of compound microscope. Antiproliferative activity was determined by using yeast cell model in which light microscope with hemocytometer was used for cell counting. In case of Antiproliferative activity, the ethyl acetate extract of <jats:italic>A. aspera<\/jats:italic> had highest antiproliferative activity with lowest cell viability (22.14\u2009\u00b1\u20090.076%) at highest extract concentration (2\u2009mg\/mL) while methanol extract of <jats:italic>A. aspera<\/jats:italic> had highest antiproliferative activity with lower cell viability (24.24\u2009\u00b1\u20090.057%) at lowest extract concentration (0.25\u2009mg\/mL). The results of the study indicated that the leaves extract of <jats:italic>A. aspera<\/jats:italic> have strong potential to be used as a source of anti\u2010cancer agent.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:italic>Achyranthes aspera<\/jats:italic> L. leaves have various phytochemicals which contribute to its medicinal properties<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Various extracts of the leaves of <jats:italic>A. aspera<\/jats:italic> L. possess antioxidant, antimitotic and antiproliferative potential<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results of the study indicated that the leaves extract of <jats:italic>A. aspera<\/jats:italic> have strong potential to be used as a source of anti\u2010cancer agent.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24495","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T05:00:01Z","timestamp":1704949201000},"page":"1031-1043","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Application of microscopy and spectroscopy in investigating anti\u2010cancer potential of <i>Achyranthes aspera<\/i> L. leaves"],"prefix":"10.1002","volume":"87","author":[{"given":"Huma","family":"Bashir","sequence":"first","affiliation":[{"name":"Department of Biological Sciences University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7805-8611","authenticated-orcid":false,"given":"Sehrish","family":"Sadia","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeb","family":"Saddiqe","sequence":"additional","affiliation":[{"name":"Department of Botany Govt. Queen Mary Graduate College  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mubashrah","family":"Munir","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences University of Veterinary and Animal Sciences  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohang","family":"Bai","sequence":"additional","affiliation":[{"name":"School of Architecture Southeast University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiyu","family":"Jia","sequence":"additional","affiliation":[{"name":"East China University of Technology  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khawaja Shafique","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Botany University of Poonch  Rawalakot Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.15415\/jptrm.2019.72009"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2007.04.014"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2012.657766"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/129697"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(01)00137-1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470089941.et0603s08"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf00044a048"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0255937"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e15909"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3732"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.36899\/JAPS.2022.3.0475"},{"key":"e_1_2_8_13_1","first-page":"49","volume-title":"Phytochemical methods\u2010a guide to modern techniques of plant analysis","author":"Harborne J. 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