{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:11:05Z","timestamp":1792811465066,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T00:00:00Z","timestamp":1707868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Diabetes is a life\u2010threatening disease that affects different parts of the body including the liver, kidney, and pancreas. The core root of diabetes is mainly linked to oxidative stress produced by reactive oxygen species (ROS).\n                      <jats:italic>Berberis lyceum<\/jats:italic>\n                      Royle (BLR) is the source of natural products. It comprises numerous bioactive compounds having antioxidant activities. In the current investigation, silver nanoparticles from BLR root extract\u00a0were synthesized, characterized, and assessed for antidiabetic potential. UV spectrophotometry, Transmission electron microscopy (TEM), Fourier transform infra\u2010red spectroscopy (FTIR), and x\u2010ray diffraction (XRD) were applied for the characterization of NPs. It was evident from the morphological studies that the synthesized NPs were spherical and the average size was 11.02\u2009nm. Results revealed that BLR\u2010AgNPs showed higher radical scavenging activity as compared to BLR extract. Moreover, BLR\u2010AgNPs displayed superior in vivo and in vitro antidiabetic activity in comparison to BLR extract. Glucose level (116.5\u2009\u00b1\u20095.1\u2009mg\/dL), liver function test (ALAT: 54.038\u2009\u00b1\u20096.2\u2009IU\/L; ASAT: 104.42\u2009\u00b1\u200913.9\u2009IU\/L; ALP: 192.6\u2009\u00b1\u20092.4\u2009IU\/L; bilirubin: 1.434\u2009\u00b1\u20090.14\u2009mg\/dL; total protein: 5.14\u2009\u00b1\u20090.24\u2009mg\/dL), renal function test (urea: 39.6\u2009\u00b1\u20090.63\u2009mg\/dL; uric acid: 21.4\u2009\u00b1\u20090.94\u2009mg\/dL; creatinine: 0.798\u2009\u00b1\u20090.03\u2009mg\/dL; albumin: 4.14\u2009\u00b1\u20090.2\u2009mg\/dL), lipid profile level (cholesterol: 101.62\u2009\u00b1\u20093\u2009mg\/dL; triglyceride: 110.42\u2009\u00b1\u20097\u2009mg\/dL; HDL\u2010C: 29.7\u2009\u00b1\u20093\u2009mg\/dL; LDL\u2010C: 47.056\u2009\u00b1\u20091\u2009mg\/dL; VLDL\u2010C: 22.0\u2009\u00b1\u20091.3\u2009mg\/dL) and hematology (WBCs: 3.82\u2009\u00b1\u20090.24 10\n                      <jats:sup>3<\/jats:sup>\n                      \/\u03bcL; RBCs: 4.78\u2009\u00b1\u20090.42 10\n                      <jats:sup>6<\/jats:sup>\n                      \/\u03bcL; Hb: 12.6\u2009\u00b1\u20091.0\u2009g\/dL; Hematocrit: 39.4\u2009\u00b1\u20093.7%; MCV: 65.8\u2009\u00b1\u20093\u2009fL; platelets: 312\u2009\u00b1\u200922.4; neutrophils: 34.8\u2009\u00b1\u20091.87; eosinophils: 3.08\u2009\u00b1\u20090.43; monocytes: 3.08\u2009\u00b1\u20090.28; lymphocytes: 75.6\u2009\u00b1\u20093.77) confirmed the significant antidiabetic potential of BLR\u2010AgNPs. Histopathological examination authenticated\u00a0that BLR\u2010AgNPs caused\u00a0a significant revival in the morphology of the liver, kidney, and pancreas. Hence, findings of the study suggested the\u00a0BLR\u2010AgNPs as a potent antidiabetic agent\u00a0and could be an appropriate nanomedicine to prevent diabetes in future.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Research Highlights<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:italic>Berberis lyceum<\/jats:italic>\n                            extract as a reducing, capping, and stabilization agent for the BLR\u2010AgNPs synthesis\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Evaluation of \u03b1\u2010amylase inhibition, antioxidant, and \u03b1\u2010glucosidase inhibition potential<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            Thorough characterization using Fourier transform infrared spectroscopy, Transmission electron microscopy, x\u2010ray diffraction, and UV\u2013VIS spectrophotometer, which is 1\n                            <jats:sup>st<\/jats:sup>\n                            of its kind\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>In\u2010vivo antidiabetic activity evaluation through multiple biomarkers<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jemt.24509","type":"journal-article","created":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T03:33:55Z","timestamp":1707881635000},"page":"1286-1305","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Evaluating the biological (antidiabetic) potential\u00a0of\n                    <scp>TEM<\/scp>\n                    ,\n                    <scp>FTIR<\/scp>\n                    ,\n                    <scp>XRD<\/scp>\n                    , and\n                    <scp>UV<\/scp>\n                    \u2010spectra observed\n                    <i>berberis lyceum<\/i>\n                    conjugated silver nanoparticles"],"prefix":"10.1002","volume":"87","author":[{"given":"Tafail Akbar","family":"Mughal","sequence":"first","affiliation":[{"name":"Department of Zoology Women University of Azad Jammu &amp; Kashmir  Bagh Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaukat","family":"Ali","sequence":"additional","affiliation":[{"name":"Medical Toxicology and Biochemistry Laboratory, Department of Zoology Government College University  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumaila","family":"Mumtaz","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Poonch  Rawalakot Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Summer","sequence":"additional","affiliation":[{"name":"Medical Toxicology and Biochemistry Laboratory, Department of Zoology Government College University  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Zubair","family":"Saleem","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Natural Medicine Pharmacology, School of Pharmacy Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Hassan","sequence":"additional","affiliation":[{"name":"Medical Toxicology and Biochemistry Laboratory, Department of Zoology Government College University  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Usman","family":"Hameed","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Poonch  Rawalakot Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,14]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","first-page":"14","article-title":"Amelioration of altered serum, liver, and kidney antioxidant enzymes activities by sodium selenite in alloxan\u2010induced diabetic rats","volume":"3","author":"Ahmadvand H.","year":"2014","journal-title":"Reports Of Biochemistry And Molecular Biology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2015.02.007"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/1519-6984.185979"},{"issue":"1","key":"e_1_2_9_5_1","first-page":"4","article-title":"General approaches to the stem cell therapy in diabetes mellitus as innovative researches","volume":"1","author":"Alp E. 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