{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T14:13:04Z","timestamp":1778249584646,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Wear particle\u2010induced periprosthetic osteolysis is the key to aseptic loosening after artificial joint replacement. Osteoclastogenesis plays a central role in this process. Apelin\u201013 is a member of the adipokine family with anti\u2010inflammatory effects. Here, we report that apelin\u201013 alleviates RANKL\u2010mediated osteoclast differentiation and titanium particle\u2010induced osteolysis in mouse calvaria. Mechanistically, apelin\u201013 inhibits NLRP3 inflammasome\u2010mediated pyroptosis by activating the nuclear factor erythroid 2\u2010related factor 2 (Nrf2) pathway. In summary, apelin\u201013 is expected to be a potential drug for relieving aseptic osteolysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>This study reveals the molecular mechanism by which apelin\u201013 inhibits NLRP3 inflammasome activation and pyroptosis by promoting Nrf2.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>This study confirms that apelin\u201013 alleviates osteoclast activation by inhibiting pyroptosis.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In vivo studies further confirmed that apelin\u201013 alleviated mouse skull osteolysis by inhibiting the activation of NLRP3 inflammasome.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24519","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T05:35:57Z","timestamp":1708493757000},"page":"1348-1358","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Apelin\u201013 alleviates osteoclast formation and osteolysis through Nrf2\u2010pyroptosis pathway"],"prefix":"10.1002","volume":"87","author":[{"given":"Zhaoyang","family":"Yin","sequence":"first","affiliation":[{"name":"Department of Orthopedics The Affiliated Lianyungang Hospital of Xuzhou Medical University (The First People's Hospital of Lianyungang)  Lianyungang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinghua","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Nanjing Lishui People's Hospital, Zhongda Hospital Lishui Branch Southeast University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Affiliated Jiangning Hospital with Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Affiliated Jiangning Hospital with Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6435-775X","authenticated-orcid":false,"given":"Jian","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Affiliated Jiangning Hospital with Nanjing Medical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Affiliated Jiangning 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