{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T03:24:15Z","timestamp":1789269855264,"version":"build-2803163510"},"reference-count":65,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Coccidiosis is a protozoan parasitic disease caused by <jats:italic>Eimeria<\/jats:italic> species and affects wild and domestic animals. Coccidiostats are currently available to control this disease, although drug resistance has been confirmed for all of them. As a result, there is an urgent need to identify eco\u2010friendly agents to control and treat this disease. This study aimed to investigate the ameliorative role of the <jats:italic>Krameria lappacea<\/jats:italic> roots extract (KLRE) on the outcome of coccidiosis induced by <jats:italic>Eimeria papillata<\/jats:italic>. Male C57BL\/6 mice were divided into seven groups (5 mice\/group), as follows: Group 1: noninfected\u2010nontreated (control group), Group 2: noninfected\u2010treated group with KLRE (200\u2009mg\/kg), Group 3: infected\u2010nontreated group, Group 4: infected\u2010treated group with KLRE (50\u2009mg\/kg), Group 5: infected\u2010treated group with KLRE (100\u2009mg\/kg), Group 6: infected\u2010treated group with KLRE (200\u2009mg\/kg), and Group 7: infected\u2010treated group with amprolium (120\u2009mg\/kg). Groups (3\u20137) were inoculated orally with 1\u2009\u00d7\u200910<jats:sup>3<\/jats:sup> sporulated <jats:italic>E. papillata<\/jats:italic> oocysts. One hour after infection, groups (4\u20136) were daily treated for 5\u2009days with KLRE and amprolium. On day 5 postinfection, oocyst output was determined, and mice were euthanized for the collection of jejuna then preparation of histological sections and jejunal homogenate was used for the determination of biochemical and oxidative damage markers. The coccidial infection induced weight loss of mice by 3.971%, which improved after KLRE to \u22121.512%. After KLRE treatment, the rate of feed intake was improved to be 52.21\u2009\u00b1\u20092.30 than those in infected group (40.47\u2009\u00b1\u20092.25). Oocyst output was significantly reduced in mice treated with KLRE (1.308\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> oocysts\/g.feces) compared with those in the infected group (5.387\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> oocysts\/g.feces). <jats:italic>E. papillata<\/jats:italic> infection induced marked histological alterations within jejunum tissue. After treatment, KLRE was able to impair the development of parasite stages (meronts, gamonts, and developing oocysts) in the jejunum through a significant reduction of number and size in comparison with the infected group. Infection with <jats:italic>E. papillata<\/jats:italic> induced a disturbance in the nutrient absorption in the jejunal mice tissue, which improved after the treatment with KLRE and amprolium. Also, KLRE counteracted significantly the <jats:italic>E. papillata<\/jats:italic>\u2010induced loss of reduced glutathione and total antioxidant capacity. Our findings indicate that KLRE could be used as an alternative to the available coccidiostats currently available.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:italic>Krameria lappacea<\/jats:italic> exhibit significant anticoccidial and antioxidant activities induced by <jats:italic>E. papillata<\/jats:italic> infection.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:italic>Krameria lappacea<\/jats:italic> exhibit significant improvement in the pathological alterations of the jejunal tissue induced by <jats:italic>E. papillata<\/jats:italic> infection.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24531","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T07:09:14Z","timestamp":1708931354000},"page":"1467-1478","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Anticoccidial and jejunum\u2010protective effects of <i>Krameria lappacea<\/i> roots extract on experimental <i>Eimeria papillata<\/i> infection"],"prefix":"10.1002","volume":"87","author":[{"given":"Ghada","family":"Alamari","sequence":"first","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0633-930X","authenticated-orcid":false,"given":"Rewaida","family":"Abdel\u2010Gaber","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esam M.","family":"Al\u2010Shaebi","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saleh","family":"Al\u2010Quraishy","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,26]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"61","article-title":"Comparative efficacy of selected anticoccidial drugs in ambarorow sheep naturally infected with enteric coccidia in South Darfur, Sudan","volume":"20","author":"Abakar A. D.","year":"2005","journal-title":"The Sudan Journal of Veterinary Research"},{"issue":"1","key":"e_1_2_10_3_1","first-page":"44","article-title":"In vitro anticoccidial activity of Trachyspermum ammi (Ajwain) extract on oocysts of Eimeria species of chicken","volume":"7","author":"Abbas A.","year":"2019","journal-title":"Advanced Life Sciences"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1139899"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-5035-0"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11686-020-00219-w"},{"key":"e_1_2_10_7_1","volume-title":"Arbeitsmethoden der makroskopischen und mikroskopischen anatomic Mit 283 Abbildungen Gustav","author":"Adam H.","year":"1964"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.22-0136"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/0001-3765202020191107"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S162355"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.1.58-65.2002"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2021.01.008"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/fst.123722"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2266-y"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2020.02.034"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1590\/s1984-29612019068"},{"key":"e_1_2_10_17_1","first-page":"409","article-title":"In vivo anti\u2010Eimeria and in vitro anthelmintic activity of Ziziphus spina\u2010christi leaf extracts Pakistan","volume":"48","author":"Alzahrani F.","year":"2016","journal-title":"Journal of Zoology"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/219670"},{"issue":"3","key":"e_1_2_10_19_1","first-page":"136","article-title":"Effects of local gametocyte and livacox vaccines on live body weight gain and lymphoid organs in chickens","volume":"28","author":"Anwar M. I.","year":"2008","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2011.06.016"},{"key":"e_1_2_10_21_1","unstructured":"Bombardelli E. Morazzoni P. Cristoni A. Seghizzi R. &Indena S. A.(2002).Pharmaceutical and cosmetic formulations with antimicrobial activity(U.S. Patent No. 6 475 536)."},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2018.00004"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2002-23167"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-407705-8.00002-1"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3430-3"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.09.009"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.11.031"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2019.02.006"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-023-25383-0"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.2307\/3284470"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(59)90090-6"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00067-12"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.15381\/ci.v10i2.4964"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10040428"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)57021-6"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1752-153X-5-33"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1298242"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-13-12"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2168-4"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.54.5.356"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbp.2010.04.008"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2012.06.029"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.5487\/TR.2017.33.4.273"},{"issue":"4","key":"e_1_2_10_44_1","first-page":"401","article-title":"Role of natural antioxidants for the control of coccidiosis in poultry","volume":"33","author":"Masood S.","year":"2013","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-27769-6"},{"issue":"15","key":"e_1_2_10_46_1","first-page":"3041","article-title":"Role of Phoenix dactylifera in ameliorating Eimeria papillata induced hepatic injury in mice","volume":"6","author":"Metwaly M. S.","year":"2012","journal-title":"Journal of Medicinal Plants Research"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11756-013-0297-9"},{"issue":"4","key":"e_1_2_10_48_1","first-page":"971","article-title":"Protective effects of palm pollen aqueous extract against Eimeria papillata induced intestinal damage in mice","volume":"47","author":"Metwaly M. S.","year":"2015","journal-title":"Pakistan Journal of Zoology"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2022.02.025"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(01)00398-1"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-019-06343-5"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2018.10.029"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.8.3165-3170.1997"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.2307\/3284157"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-962032"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X1501001118"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.2.201-222.1995"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02887080"},{"issue":"4","key":"e_1_2_10_59_1","doi-asserted-by":"crossref","DOI":"10.1590\/s1984-29612020072","article-title":"Mulberry extract as an ecofriendly anticoccidial agent: In vitro and in vivo application","volume":"29","author":"Thagfan F. A.","year":"2020","journal-title":"Revista Brasileira de Parasitologia Veterin\u00e1ria"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.2174\/1386207323666200903152811"},{"key":"e_1_2_10_61_1","first-page":"53","article-title":"In vivo anticoccidial activity of Salvadora persica root extracts","volume":"49","author":"Thagfan F. A.","year":"2017","journal-title":"Pakistan Journal of Zoology"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1177\/000456326900600108"},{"key":"e_1_2_10_63_1","first-page":"807","article-title":"Protective effect of l\u2010cysteine and glutathione on the modulated suckling rat brain plasmodium berghei ANKA after chronic exposure","volume":"108","author":"Tsakiris S.","year":"2004","journal-title":"Parasitology Research"},{"issue":"2","key":"e_1_2_10_64_1","first-page":"69","article-title":"Neolignans from Krameria ramosissima (A. Gray) S. Watson with activity against Porphyromonas gingicalis, cytotoxical and mutagenic evaluations","volume":"45","author":"Villareal\u2010Garc\u00eda L. E.","year":"2014","journal-title":"Revista Mexicana de Ciencias Farmaceuticas"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.67.311"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy12112599"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24531","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T17:44:44Z","timestamp":1760636684000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.24531"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,26]]},"references-count":65,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/jemt.24531"],"URL":"https:\/\/doi.org\/10.1002\/jemt.24531","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,26]]},"assertion":[{"value":"2023-11-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}