{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:37:13Z","timestamp":1789015033422,"version":"build-2803163510"},"reference-count":75,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Cryptosporidiosis is a global health problem threats life of immunocompromised patients. <jats:italic>Allium sativum (A. sativum)<\/jats:italic> is one of the therapeutic options for cryptosporidiosis. This study develops green synthesized ZnO\u2010NPs based on <jats:italic>A. sativum<\/jats:italic> extract, and assesses its therapeutic application in treating experimental cryptosporidiosis in immunosuppressed mice. FTIR, scanning electron microscopy, and zeta analyzer were used for characterization of bio ZnO\u2010NPs. The morphology of prepared materials appeared as sponge with many pores on the whole surface that allows the feasibility of bio ZnO\u2010NPs for different biological activities. Its structural analysis was highly stabilized with negative charge surface which indicated for well distribution into the parasite matrix. Twenty\u2010five immunosuppressed <jats:italic>Cryptosporidium parvum<\/jats:italic> infected mice, classified into 5 groups were sacrificed at 21th day after infection with evaluation of parasitological, histopathological, oxidative, and proinflammatory biomarkers. Treated mice groups with 50 and 100\u2009mg\/kg of AS\/ZnO\u2010NPs showed a highly significant decline (79.9% and 83.23%, respectively) in the total number of expelled oocysts. Both doses revealed actual amelioration of the intestinal, hepatic, and pulmonary histopathological lesions. They also significantly produced an increase in GSH values and improved the changes in NO and MDA levels, and showed high anti\u2010inflammatory properties. This study is the first to report green synthesis of ZnO\/<jats:italic>A. sativum<\/jats:italic> nano\u2010composite as an effective therapy in treating cryptosporidiosis which gave better results than using <jats:italic>A. sativum<\/jats:italic> alone. It provides an economical and environment\u2010friendly approach towards novel delivery synthesis for antiparasitic applications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Green synthesis of ZnO\u2010NPs was developed using <jats:italic>A. sativum<\/jats:italic> extract.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The morphology of prepared ZnO\u2010NPs appeared as sponge with many pores on SEM<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The study evaluates its therapeutic efficacy against murine cryptosporidiosis<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The green synthesized ZnO\u2010NPs significantly reduced percent of oocyst shedding, improved the pathological changes, and showed high antioxidant and anti\u2010inflammatory potentials.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24541","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T05:19:12Z","timestamp":1711948752000},"page":"1912-1925","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Green synthesis of zinc oxide\/<i>Allium sativum<\/i> nano\u2010composite and its efficacy against murine cryptosporidiosis"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6235-7174","authenticated-orcid":false,"given":"Doaa A.","family":"Hamdy","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology College of Medicine, Beni\u2010Suef University  Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mousa A. 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