{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T07:36:16Z","timestamp":1768462576745,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T00:00:00Z","timestamp":1709337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p><jats:italic>Trichinella spiralis<\/jats:italic> infection is a food\u2010borne zoonotic disease caused by nematodes that dwell in the tissues, presenting a significant public health concern. This study aimed to evaluate the effectiveness of different treatments including silver nanoparticles (AgNPs), myrrh biosynthesized AgNPs \u201cAgNPs synthesized using plant\u2010based green technologies\u201d, myrrh extract, and myrrh essential oil, as alternative treatments against <jats:italic>T. spiralis<\/jats:italic> infection. Parasitological, histopathological, and cytotoxicity assessments were conducted to investigate the effects of various concentrations of these treatments in reducing the populations of adult worms and larvae during both the intestinal and muscular phases of <jats:italic>T. spiralis\u2010<\/jats:italic>infected mice. The results showed that the highest antihelminthic efficacy against the intestinal phase of <jats:italic>T. spiralis<\/jats:italic> was achieved by myrrh extract (86.66%), followed closely by AgNPs (84.96%) and myrrh AgNPs (82.51%) at higher concentrations (800\u2009mg\/kg for myrrh extract, 40\u2009\u03bcg\/mL for AgNPs, and 40\u2009\u03bcg\/mL for myrrh AgNPs). While the group treated with myrrh essential oil showed the lowest percentage of adult reduction (78.14%). However, all treatments demonstrated comparable effects in reducing the larvae population in the muscle phase. Histopathological examination of the tissues revealed compelling evidence of the effectiveness of AgNPs, particularly when prepared with myrrh. Additionally, a comprehensive assessment of the cytotoxicity of AgNPs indicated low toxicity levels. This study supports that AgNPs synthesized using plant\u2010based green technologies hold therapeutic potential for the treatment of <jats:italic>T. spiralis<\/jats:italic> infection. These findings present a promising avenue for the development of novel antiparasitic drugs that are both effective and safe.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Myrrh extract has the highest antihelminthic efficacy against the intestinal phase of <jats:italic>T. spiralis<\/jats:italic>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Histopathological examination of the tissues revealed compelling evidence of the effectiveness of AgNPs, particularly when prepared with myrrh.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>During intestinal phase of <jats:italic>T. spiralis<\/jats:italic>, varying levels of nanoparticle precipitation were detected in the liver, brain, lung, and intestine. During the muscular phase, the highest amount of AgNPs precipitation was detected in the liver, followed by the brain, and lung.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24542","type":"journal-article","created":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T07:34:01Z","timestamp":1709364841000},"page":"1566-1575","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Light microscopical and parasitological analyses revealed the beneficial effects of silver nanoparticles and various myrrh extracts against <i>Trichinella spiralis<\/i> infection in mice"],"prefix":"10.1002","volume":"87","author":[{"given":"Nahed A.","family":"Elossily","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology, Faculty of Medicine Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salwa M.","family":"Abd\u2010ELrahman","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abeer A.","family":"Khedr","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine New Valley University  New Valley Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed K.","family":"Dyab","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Faculty of Medicine Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abeer E.","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Faculty of Medicine Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaymaa M.","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed M.","family":"Abd Elrahman","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fahd M.","family":"Alsharif","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics and Ind. Pharmacy College of Pharmacy, Al\u2010Azhar University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reem M.","family":"Alsaadawy","sequence":"additional","affiliation":[{"name":"Department of Zoonoses, Faculty of Veterinary Medicine Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4467-6742","authenticated-orcid":false,"given":"Ramy K. A.","family":"Sayed","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine Sohag University  Sohag Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mervat M.","family":"Khalifa","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Faculty of Medicine Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,2]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"591","article-title":"Influence of chemically and biosynthesized silver nanoparticles on in vitro viability and infectivity of Trichinella spiralis muscle larvae","volume":"67","author":"Abd\u2010Elrahman S. 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