{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:34:30Z","timestamp":1760661270104,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021565","name":"Montpellier Universit\u00e9 d'Excellence","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021565","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Detecting RNA molecules within their natural environment inside intact arthropods has long been challenging, particularly in small organisms covered by a tanned and pigmented cuticle. Here, we have developed a methodology that enables high\u2010resolution analysis of the spatial distribution of transcripts of interest without having to dissect tiny organs or tissues, thereby preserving their integrity. We have combined an in situ amplification approach based on hybridization chain reaction, which enhances the signal\u2010to\u2010noise ratio, and a clearing approach that allows the visualization of inner organs beneath the cuticle. We have implemented this methodology for the first time in Hemiptera, mapping two salivary aphid (<jats:italic>Acyrthosiphon pisum<\/jats:italic>) transcripts, the effector c002 and the salivary sheath protein SHP. With a multiplex approach, we could simultaneously detect different mRNAs in mounted pea aphid head\u2010thorax samples and show that they were distributed in distinct secretory cells of salivary glands.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Combining hybridisation chain reaction and clearing allows the detection of transcripts in intact aphids heads.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The transcripts of the two salivary proteins c002 and SHP are compartmentalized in distinct secretory cells of the principal glands.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24561","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T06:39:28Z","timestamp":1711953568000},"page":"1926-1932","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Coupling clearing and hybridization chain reaction approaches to investigate gene expression in organs inside intact insect heads"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4645-8709","authenticated-orcid":false,"given":"Bastien","family":"Cayrol","sequence":"first","affiliation":[{"name":"PHIM Plant Health Institute, Univ Montpellier, INRAE, CIRAD, Institut Agro, IRD  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3139-6055","authenticated-orcid":false,"given":"Stefano","family":"Colella","sequence":"additional","affiliation":[{"name":"PHIM Plant Health Institute, Univ Montpellier, INRAE, CIRAD, Institut Agro, IRD  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7459-8434","authenticated-orcid":false,"given":"Marilyne","family":"Uzest","sequence":"additional","affiliation":[{"name":"PHIM Plant Health Institute, Univ Montpellier, INRAE, CIRAD, Institut Agro, IRD  Montpellier France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2010.04.001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr100881q"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800692"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn405717p"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.140137"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.165753"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0238134"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407024101"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.asd.2019.100883"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.11.016"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.07.024"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.694"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-010-1099-9"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20209807"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708958105"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1673\/031.006.3801"},{"issue":"72","key":"e_1_2_9_18_1","first-page":"1","article-title":"The site of potato leafroll virus multiplication in its vector, Myzus persicae\u2009: An anatomical study","volume":"16","author":"Ponsen M. 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