{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:50:38Z","timestamp":1792518638519,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T00:00:00Z","timestamp":1718582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>This study presents a comprehensive investigation into the evolutionary trajectories of <jats:italic>Rhipicephalus<\/jats:italic> ticks (Ixodidae) through the interpretation of molecular phylogenetics, elucidating their chromatographic spectrum. The use of advanced chromatographic tools in this study explored the dynamics chemical profiling, providing valuable insights into the evolutionary history and ecological adaptations. Prevalence of <jats:italic>Rhipicephalus<\/jats:italic> ticks was 4.5% in sheep and 3.9% in goats. The ITS2 sequence of the <jats:italic>Rhipicephalus sanguineus<\/jats:italic> (OK642408) and <jats:italic>Rhipicephalus microplus<\/jats:italic> (OK642409) form a distinct clade with sequences from other countries. The 16S rRNA sequences of <jats:italic>R. sanguineus<\/jats:italic> (OK560870) clustered with sequences form three lineages, tropical, temperate, and south\u2010eastern. The Cox I gene\u2010identified <jats:italic>Rhipicephalus turanicus<\/jats:italic> (OK623472) and <jats:italic>R. microplus<\/jats:italic> (OK623463) form separate clades with sequences. The HPLC chromatogram of tick samples reveals a diverse array of identified hydrocarbons, explained the complex chemical composition of their exoskeletons. This analytical approach provides valuable insights into the specific hydrocarbon profiles, allowing for potential applications in species differentiation, ecological studies, and a deeper understanding of the functional roles played by hydrocarbon compounds in tick physiology. The findings revealed the potential of applying molecular phylogenetics tools with chromatography not only to enhance our understanding of tick evolution but also to inform strategies for disease control and management in regions where <jats:italic>Rhipicephalus<\/jats:italic> ticks (Ixodidae) are endemic.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Chemical mapping utilizing advanced chromatographic techniques.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Scanning microscopic insights high\u2010resolution scanning tool to observe structural and morphological features of ticks at a molecular level.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Molecular phylogeny data elucidate the evolutionary relationships among tick species.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24620","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T07:29:16Z","timestamp":1718609356000},"page":"2590-2602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Revealing tick diversity: Chemical profiling and dynamics in scanning microscopy and molecular phylogenetics"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2400-174X","authenticated-orcid":false,"given":"Ayesha","family":"Malik","sequence":"first","affiliation":[{"name":"Department of Zoology, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiran","family":"Afshan","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad K.","family":"Okla","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology College of Science, King Saud University Riyadh  Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim A.","family":"Saleh","sequence":"additional","affiliation":[{"name":"Faculty of Science Zarqa University  Zarqa Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Razzaq","sequence":"additional","affiliation":[{"name":"Animal Sciences Division Pakistan Agricultural Research Council  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munib","family":"Hussain","sequence":"additional","affiliation":[{"name":"Animal Sciences Division Pakistan Agricultural Research Council  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabika","family":"Firasat","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Biological Sciences Quaid\u2010i\u2010Azam University Islamabad  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erinda","family":"Lika","sequence":"additional","affiliation":[{"name":"Department of Preclinical Subject, Faculty of Veterinary Medicine Agricultural University of Tirana  Tirana Albania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e0rius Vicent","family":"Fuentes","sequence":"additional","affiliation":[{"name":"Departament de Parasitologia, Facultat de Farm\u00e0cia Universitat de Val\u00e8ncia  Val\u00e8ncia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00580-018-2786-2"},{"key":"e_1_2_11_3_1","doi-asserted-by":"crossref","unstructured":"Amer A. 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