{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T10:15:31Z","timestamp":1787912131917,"version":"build-2784847793"},"reference-count":68,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T00:00:00Z","timestamp":1720915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100016047","name":"Science Fund of the Republic of Serbia","doi-asserted-by":"publisher","award":["7394"],"award-info":[{"award-number":["7394"]}],"id":[{"id":"10.13039\/501100016047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016047","name":"Science Fund of the Republic of Serbia","doi-asserted-by":"publisher","award":["7749444"],"award-info":[{"award-number":["7749444"]}],"id":[{"id":"10.13039\/501100016047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Skeletal porous lesions such as <jats:italic>cribra orbitalia<\/jats:italic> (CO) have long been of interest to bioanthropologists worldwide, mainly due to their high prevalence in osteological material. Previous studies considered CO as an external morphological manifestation, and therefore, research has mainly focused on visible (macroscopic) CO patterns. However, the understanding of CO\u2010induced micro\u2010scale bone changes is still scarce. Therefore, we performed high\u2010resolution micro\u2010computed tomography imaging to investigate three\u2010dimensional CO\u2010induced micro\u2010architectural patterns in non\u2010adults, with a particular focus on the correlation between macroscopic and micro\u2010architectural orbital features. Cortical and trabecular micro\u2010architectural changes in the orbital roof were analyzed in non\u2010adults younger than 15\u2009years, using orbital roof samples with and without macroscopic traces of CO (<jats:italic>n<\/jats:italic>\u2009=\u200928). A widely accepted five\u2010grade macroscopic CO scoring system was applied to analyze CO severity. Areas affected with CO (area 1) and areas without macroscopic CO traces (area 2) were analyzed separately. The conducted high\u2010resolution analysis showed that cortical and trabecular micro\u2010architecture varied with CO presence, lesion severity (CO grade), and the analyzed area. Inter\u2010grade comparisons suggested that most of the analyzed micro\u2010architectural parameters were not significantly different between adjacent CO grades. Based on the micro\u2010architectural evaluation of areas 1 and 2, the porous lesions were much more extensive than revealed by gross examination. In addition, micro\u2010architectural differences were particularly pronounced in younger non\u2010adults. In summary, our pilot study suggests that the macroscopic examination of CO reflects only <jats:italic>the tip of the iceberg<\/jats:italic>, as the micro\u2010architectural changes seem to be much larger than macroscopically identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:italic>Cribra orbitalia<\/jats:italic> (CO) represents orbital porous lesions.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>A high\u2010resolution microscopic assessment of CO\u2010induced changes in non\u2010adults was done by micro\u2010computed tomography.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The microarchitecture was affected by CO presence, CO grade, area, and age.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24656","type":"journal-article","created":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T15:19:06Z","timestamp":1720970346000},"page":"2818-2828","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["High\u2010resolution three\u2010dimensional micro\u2010computed tomography assessment of micro\u2010architectural patterns in non\u2010adults with <i>cribra orbitalia<\/i>: Correlation between macro\u2010 and micro\u2010scale bone features"],"prefix":"10.1002","volume":"87","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0326-1949","authenticated-orcid":false,"given":"Veda","family":"Mikasinovic","sequence":"first","affiliation":[{"name":"Center of Bone Biology, Institute of Anatomy, Faculty of Medicine University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7637-4357","authenticated-orcid":false,"given":"Ksenija","family":"Djukic","sequence":"additional","affiliation":[{"name":"Center of Bone Biology, Institute of Anatomy, Faculty of Medicine University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3941-270X","authenticated-orcid":false,"given":"Petar","family":"Milenkovic","sequence":"additional","affiliation":[{"name":"Center of Bone Biology, Institute of Anatomy, Faculty of Medicine University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3124-1400","authenticated-orcid":false,"given":"Jelena","family":"Jadzic","sequence":"additional","affiliation":[{"name":"Center of Bone Biology, Institute of Anatomy, Faculty of Medicine University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1461-8437","authenticated-orcid":false,"given":"Petar","family":"Milovanovic","sequence":"additional","affiliation":[{"name":"Center of Bone Biology, Institute of Anatomy, Faculty of Medicine University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6267-827X","authenticated-orcid":false,"given":"Marija","family":"Djuric","sequence":"additional","affiliation":[{"name":"Center of Bone Biology, Institute of Anatomy, Faculty of Medicine University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,14]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Anderson A.(2022).Skeletal indicators of early life stress: Insights into cribra orbitalia and porotic hyperostosis in a living subsistence population. PhD thesis University of California Santa Barbara."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpp.2021.04.008"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.153.3737.760"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1556-4029.13131"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.10431"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510377791"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.23701"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-101020-4.00002-1"},{"key":"e_1_2_10_10_1","volume-title":"Digging up bones","author":"Brothwell D. R.","year":"1981"},{"key":"e_1_2_10_11_1","volume-title":"Ortner's identification of pathological conditions in human skeletal remains","author":"Buikstra J. E.","year":"2019"},{"key":"e_1_2_10_12_1","unstructured":"Buikstra J. E. &Ubelaker D. H.(1994).Standards for data collection from human skeletal remains research series No. 4. Arkansas Archaeological Survey."},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/26\/3\/003"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.24038"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/oa.955"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1700940205"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0940-9602(04)80035-9"},{"key":"e_1_2_10_18_1","unstructured":"Galea J. C.(2013).Analysing the microarchitecture of cribra orbitalia via micro computed tomography in post\u2010medieval remains from the Bristol Royal Infirmary[MPhil thesis University of Bristol England]."},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20778"},{"key":"e_1_2_10_20_1","first-page":"57","article-title":"Cribra orbitalia: Pathogenesis and probable etiology","volume":"22","author":"Hengen O. P.","year":"1971","journal-title":"HOMO\u2014Journal of Comparative Human Biology"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0213369"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/oa.619"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00361188"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchb.2006.11.002"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.12659\/pjr.883628"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1001\/archpedi.1955.02050110865010"},{"key":"e_1_2_10_27_1","first-page":"157","volume-title":"Human growth and development","author":"Maresh M. M.","year":"1970"},{"key":"e_1_2_10_28_1","first-page":"1","article-title":"Micronutrient deficiency diseases: Anemia, scurvy, and rickets","volume":"1","author":"Mays S.","year":"2018","journal-title":"The International Encyclopedia of Biological Anthropology"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.24091"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/01459740.1978.9986939"},{"key":"e_1_2_10_31_1","first-page":"29","volume-title":"Bioarheologija na Balkanu. Studije iz antropologije i zooarheologije","author":"Mika\u0161inovi\u0107 V.","year":"2023"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.1330930302"},{"key":"e_1_2_10_33_1","unstructured":"Morgan J. A.(2014).The methodological and diagnostic applications of micro\u2010CT to palaeopathology: A quantitative study of porotic hyperostosis[PhD thesis Electronic Thesis and Dissertation Repository]. 2056."},{"issue":"2","key":"e_1_2_10_34_1","first-page":"171","article-title":"\"Cribra orbitalia\". A bone condition of the orbit of unknown nature. Anatomical study with etiological considerations","volume":"2","author":"Nathan H.","year":"1966","journal-title":"Israel Journal of Medical Sciences"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3109\/01676830.2012.723785"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-8703(64)90235-2"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.18485\/gsad.2021.37.7"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.23902"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajhb.23896"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.24131"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.171.1.2928520"},{"key":"e_1_2_10_42_1","volume-title":"Identification of pathological conditions in human skeletal remains","author":"Ortner D. J.","year":"2003"},{"key":"e_1_2_10_43_1","unstructured":"PSLMEET2023. (2023).International meeting on porous skeletal lesions.Recommendations for PSL Recording. Retrieved February 20 2024 fromhttps:\/\/ucpages.uc.pt\/events\/pslmeet2023\/post-meeting\/"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781444345940.ch13"},{"key":"e_1_2_10_45_1","volume-title":"Diagnosis of bone and joint disorders","author":"Resnick D. R.","year":"1988"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12520-019-00780-0"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.23258"},{"key":"e_1_2_10_48_1","unstructured":"Robertson S.(2017).Cribra orbitalia in an archaeological context: New insights from micro\u2010CT analysis of the Christchurch Spitalfields collection[PhD thesis Australian National University]."},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24561"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.20611"},{"key":"e_1_2_10_51_1","volume-title":"Juvenile osteology: A laboratory and field manual","author":"Schaffer M.","year":"2009"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpp.2021.10.003"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.10024.abs"},{"key":"e_1_2_10_54_1","doi-asserted-by":"crossref","unstructured":"Schultz M. (1997). Microscopic investigation of excavated skeletal remains: A contribution to paleopathology and forensic medicine. In: Haglund W.D. Sorg M.H. editors. Forensic taphonomy. The postmortem fate of human remains p. 201\u2013222.","DOI":"10.1201\/9781439821923.ch14"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02015347"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/ca.22734"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.1330660407"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.1330740409"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.1330740410"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.1330800206"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.1330870105"},{"key":"e_1_2_10_62_1","first-page":"20","article-title":"\u00dcber Welker's cribra orbitalia","volume":"16","author":"Toldt C.","year":"1886","journal-title":"Mitteilungen der Anthropolischen Gesellschaft Wien"},{"key":"e_1_2_10_63_1","volume-title":"Human skeletal remains: Excavation, analysis, interpretation","author":"Ubelaker D. H.","year":"1984"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.21031"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpp.2023.11.001"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajpa.10321"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpp.2013.05.001"},{"key":"e_1_2_10_68_1","first-page":"1","article-title":"Cribra orbitalia, ein ethnologisch diagnostisches Merkmal am Sch\u00e4del mehrerer Menschenrassen","volume":"17","author":"Welcker H.","year":"1888","journal-title":"Architectural Anthropology"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24825"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24656","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:24:36Z","timestamp":1760624676000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.24656"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,14]]},"references-count":68,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/jemt.24656"],"URL":"https:\/\/doi.org\/10.1002\/jemt.24656","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,14]]},"assertion":[{"value":"2024-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}