{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:23:07Z","timestamp":1760660587123,"version":"build-2065373602"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p><jats:italic>Bartonella<\/jats:italic> species (<jats:italic>Bartonella<\/jats:italic> spp.) have gained recognition as a significant human pathogen, implicated in a wide range of diseases. Among these, <jats:italic>Bartonella henselae<\/jats:italic> infection has been extensively studied for its primary occurrence in cats and its role in the development of cat\u2010scratch disease in humans. While light microscopy and transmission electron microscopy (TEM) have traditionally played crucial roles in identifying causative agents of infectious diseases, including <jats:italic>Bartonella<\/jats:italic> spp., the accuracy of these methods in identifying <jats:italic>Bartonella<\/jats:italic> spp. remains undefined. Therefore, this study aims to bridge this gap by employing both light microscopy and TEM to detect <jats:italic>Bartonella<\/jats:italic> in feline blood samples and to confirm <jats:italic>B. henselae<\/jats:italic> with polymerase chain reaction (PCR). Examination of blood smears stained with Giemsa and toluidine blue semithin sections by using light microscopy revealed the presence of intraerythrocytic corpuscles, suggesting <jats:italic>Bartonella<\/jats:italic> infection in six out of 33 examined cat blood samples. TEM findings corroborated these observations, showcasing the engulfment of bacteria by the erythrocyte membrane, along with the presence of some <jats:italic>Bartonella<\/jats:italic> spp., adhering to the erythrocyte wall. PCR\u2010based molecular detection confirmed the presence of <jats:italic>B. henselae<\/jats:italic> in these six samples. It is concluded that light microscopy and TEM are considered valuable in the screening of cats' blood for the potential presence of <jats:italic>Bartonella<\/jats:italic>. However, further molecular techniques are essential for precise identification and confirmation of specific <jats:italic>Bartonella<\/jats:italic> spp.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Giemsa\u2010stained blood smear and semithin section showed potential intraerythrocytic <jats:italic>Bartonella<\/jats:italic> spp. corpuscles.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>TEM demonstrated the engulfment of <jats:italic>Bartonella<\/jats:italic> spp. by the erythrocyte membrane, along with the presence of some <jats:italic>Bartonella<\/jats:italic> spp. adhering to the erythrocyte wall.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Molecular analysis of blood samples from cats by PCR unveiled that six out of 33 (18.18%) samples tested positive for <jats:italic>B. henselae<\/jats:italic> infection.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24685","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T10:59:09Z","timestamp":1727261949000},"page":"279-285","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of <i>Bartonella henselae<\/i> in feline erythrocytes in Egypt by using Giemsa staining, transmission electron microscopy, and polymerase chain reaction"],"prefix":"10.1002","volume":"88","author":[{"given":"Reem M.","family":"Alsaadawy","sequence":"first","affiliation":[{"name":"Department of Zoonoses, Faculty of Veterinary Medicine Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amal S. M.","family":"Sayed","sequence":"additional","affiliation":[{"name":"Department of Zoonoses, Faculty of Veterinary Medicine Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magda M.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine Assiut University  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sary Kh.","family":"Abd\u2010Elghaffar","sequence":"additional","affiliation":[{"name":"Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine Assiut University  Assiut Egypt"},{"name":"School of Veterinary Medicine Badr University in Assiut  Assiut Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00521-016-2474-6"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0147-9571(96)00025-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-2712(02)00017-3"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2654.1"},{"volume-title":"Theory and Practice of Histological Techniques","year":"2008","author":"Bancroft J. 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