{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T16:44:50Z","timestamp":1783961090080,"version":"3.55.0"},"reference-count":67,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Microplastics (MPs) are small pieces of plastic that are widely distributed in the environment and accumulate within living organisms, so they are the most common types of pollutants at the present time. One of the most widespread types of MP in the environment is polyethylene (PE) MPs. There have been many published studies on the effect of PE MPs combined with other pollutants or chemicals such as benzoanthracene, emamectin benzoate, heavy metals and 4\u2010nonylphenol, on some marine, amphibian, and mouse models. However, research has rarely been conducted on how single\u2010use PE MPs affect the ileum of mammals. The current study is focused on the impact of PE MP exposure with different concentration (6, 60, 600\u2009\u03bcg\/mL PE\/MPs) for 15\u2009days, followed by 15\u2009days of recovery on small intestine(ileum) of C57BL\/6 murine model with precision and detail at the cell level by using different technique (histology, histochemistry, immunohistochemistry, and transmission electron microscope). Results demonstrated that the intestinal tissue exhibited nuclear pyknosis, villus deformation, shortness of villi, degeneration of lamina propria, hyperplasia of goblet cells, increase of goblet cells secretion, Alcian blue and Periodic acid\u2013Schiff stain positivity of intact goblet cells, highly significance of P53 immunoreaction expression specially in high concentrations (600\u2009\u03bcg\/day of PE\/MPs) and Ki\u201067 immunoreaction expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Research Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Different doses of microplastics (MPs) induced sever morphological alternations and clinical observations.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>MPs were deposits in cells and were observed in ultrastructure study.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Recovery period able to ameliorate to the most extent the alternations caused by MPs administration.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/jemt.24696","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T08:55:48Z","timestamp":1727168148000},"page":"251-269","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Microplastics induced ileum damage: Morphological and immunohistochemical study"],"prefix":"10.1002","volume":"88","author":[{"given":"Shaimaa M. 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