{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T18:06:13Z","timestamp":1788285973926,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Fluorescence in situ hybridization (FISH) has revolutionized molecular cytogenetic analysis since the 1980s, enabling precise localization of DNA sequences in cells and tissues. Despite its relevance, applying FISH to formalin\u2010fixed paraffin\u2010embedded (FFPE) tissue samples encounters significant technical challenges. This review addresses the main issues encountered in this context, such as inadequate fixation, contamination, block and slide age, inadequate pretreatment, and FISH technique. Proposed solutions include optimized pretreatment protocols, monitoring of blockage, careful selection of probes, and thorough analysis of results. Implementing good laboratory practices and quality control strategies are essential to ensure reliable results. Additionally, the use of emerging technologies such as artificial intelligence and digital pathology offers new perspectives for improving the efficiency and accuracy of FISH in FFPE samples. This review highlights the importance of a careful and personalized approach to overcome the technical challenges associated with FISH in FFPE samples, strengthening its role in research and clinical diagnosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Research Highlights<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Few FISH studies on FFPE: The scarcity of studies specifically addressing FISH applications in FFPE tissues highlights a critical gap in the literature.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Troubleshooting FISH in FFPE tissues: Identifying and addressing common challenges in FISH techniques when applied to FFPE samples, such as signal quality and hybridization efficiency.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Critical aspects of FISH technique: Discuss the main technical considerations crucial for successful FISH in FFPE tissues, including sample preparation, probe selection, and protocol optimization.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jemt.24702","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T09:00:32Z","timestamp":1727168432000},"page":"270-278","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Challenges and solutions in\n                    <scp>FISH<\/scp>\n                    for formalin\u2010fixed paraffin\u2010embedded tissue: A scoping review"],"prefix":"10.1002","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4614-5489","authenticated-orcid":false,"given":"C\u00e1ssia Campanhol","family":"Lemes","sequence":"first","affiliation":[{"name":"Research &amp; Development Laboratory Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2033-0616","authenticated-orcid":false,"given":"Andressa","family":"Germano da Silva","sequence":"additional","affiliation":[{"name":"Research &amp; Development Laboratory Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Department of Pathology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5057-4983","authenticated-orcid":false,"given":"Daniel Araki","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Department of Pathology Universidade Federal de S\u00e3o Paulo  Santos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0397-6135","authenticated-orcid":false,"given":"Andr\u00e9a Cristina de Moraes","family":"Malinverni","sequence":"additional","affiliation":[{"name":"Department of Pathology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Laboratory of Molecular and Experimental Pathology 1 Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6990-6_17"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2010.08.012"},{"key":"e_1_2_8_4_1","volume-title":"Current protocols in cell biology","author":"Bayani J.","year":"2004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/biohorizons\/hzq009"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.17305\/bjbms.2018.2519"},{"key":"e_1_2_8_7_1","volume-title":"Histotechnology a self instructional text","author":"Carson F. 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