{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T04:23:13Z","timestamp":1784262193919,"version":"3.55.0"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T00:00:00Z","timestamp":1730332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Plants are a rich source of bioactive compounds with significant pharmaceutical and health applications. This study explores the phytochemical, therapeutic, and phytotoxic properties of <jats:italic>Melia azedarach<\/jats:italic> by analyzing extracts from its bark, flowers, leaves, and fruits using six solvents: ethanol, methanol, acetone, hexane, chloroform, and distilled water. Twenty\u2010one phytochemical tests were conducted, revealing significantly positive results for various tests. However, the ethanolic and methanolic flower extracts yielded no significant results in other tests. The highest total phenolic content was found in the chloroform extract of the leaves (96\u2009\u00b1\u20090.01\u2009mg\/100\u2009g), and the highest antioxidant activity was observed in the ethanolic and hexane leaf extracts, with a 98% DPPH scavenging rate. Antibacterial testing showed significant efficacy against <jats:italic>Serratia marcescens<\/jats:italic>, <jats:italic>Bacillus subtilis<\/jats:italic>, <jats:italic>Kluyvera<\/jats:italic> spp., and <jats:italic>Pseudomonas<\/jats:italic> spp., with <jats:italic>p<\/jats:italic> values\u2009&lt;\u20090.0001. The fruit chloroform extract demonstrated the highest alpha\u2010amylase inhibition (93\u2009\u00b1\u20090.05), while the ethanolic leaf extract had the greatest tumor inhibition (85.6\u2009\u00b1\u20090.5). Insecticidal assays revealed that the acetone bark extract had the highest control values (56% and 57%). Due to their higher reducing potential, the leaves were used to biosynthesize silver nanoparticles (AgNPs), characterized by UV\u2013Vis spectroscopy, EDX, and SEM, revealing an average particle size of 20\u201330\u2009nm and spherical morphology. The AgNPs exhibited excellent antibacterial, antioxidant, antidiabetic, and insecticidal activities. These findings highlight the potential of <jats:italic>M<\/jats:italic>. <jats:italic>azedarach<\/jats:italic> and its AgNPs for developing novel therapeutic agents.<\/jats:p>","DOI":"10.1002\/jemt.24721","type":"journal-article","created":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T05:15:03Z","timestamp":1730351703000},"page":"542-554","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Synergistic Phytochemical and Nanotechnological Exploration of <i>Melia azedarach<\/i> With Silver Nitrate: Elucidating Multifaceted Antimicrobial, Antioxidant, Antidiabetic, and Insecticidal Potentials"],"prefix":"10.1002","volume":"88","author":[{"given":"Asad Husain","family":"Shah","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Faculty of Science University of Kotli  Kotli Azad Kashmir Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameena","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science University of Kotli  Kotli Azad Kashmir Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelam","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science University of Kotli  Kotli Azad Kashmir Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sammyia","family":"Jannat","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science University of Kotli  Kotli Azad Kashmir Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaloud Mohammed","family":"Alarjan","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed S.","family":"Elshikh","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afshan","family":"Afareen","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science University of Kotli  Kotli Azad Kashmir Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7785-4260","authenticated-orcid":false,"given":"Hajra","family":"Hameed","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Health Science Centre Shenzhen University  Shenzhen P. 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