{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:43:22Z","timestamp":1792115002846,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T00:00:00Z","timestamp":1739318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Silver nanoparticles (AgNPs) synthesized through green methods have garnered significant attention in nanomedicine due to their unique inorganic properties and potential for a wide range of biomedical applications, including drug delivery, wound healing, and antimicrobial therapies. This study presents a novel, eco\u2010friendly method for synthesizing AgNPs using\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Mirabilis jalapa<\/jats:italic>\n                    <\/jats:styled-content>\n                    leaf extract, which functions as both a reducing and stabilizing agent. The leaf extract, rich in bioactive compounds such as triterpenes, alkaloids, flavonoids, steroids, and phenolic compounds, plays a critical role in the reduction of silver ions (Ag\n                    <jats:sup>+<\/jats:sup>\n                    ) to form elemental AgNPs. The synthesized AgNPs were rigorously characterized using powder X\u2010ray diffraction (XRD), UV\u2013visible spectroscopy, scanning electron microscopy (SEM), and energy\u2010dispersive X\u2010ray (EDX) analysis. UV\u2013visible spectra exhibited an absorption peak in the range of 431\u2013446\u2009nm, indicative of the formation of polydispersed AgNPs with surface plasmon resonance, while XRD analysis confirmed their crystalline face\u2010centered cubic (FCC) structure. SEM imaging revealed spherical and quasi\u2010spherical morphologies, while EDX spectra confirmed the presence of silver with minimal contamination. Antibacterial activity, assessed using the agar well diffusion method, demonstrated significant inhibitory effects against Gram\u2010positive and Gram\u2010negative bacteria, although the nanoparticles showed reduced efficacy against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bacillus subtilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    . These promising antimicrobial properties, coupled with the long\u2010established use of silver in wound healing and infection management, highlight the potential of these AgNPs in biomedical applications. The novelty of this research lies in the use of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. jalapa<\/jats:italic>\n                    <\/jats:styled-content>\n                    leaf extract for the synthesis of AgNPs, offering a green and sustainable alternative to conventional chemical methods. Future studies should delve into optimizing the synthesis process for large\u2010scale production, evaluating the in\u00a0vivo efficacy of the nanoparticles, and exploring their interactions with a broader range of pathogens. This work paves the way for the development of green\u2010synthesized AgNPs as effective, eco\u2010friendly agents for infection control and wound care in nanomedicine, with the potential to significantly advance the field by offering safer, more sustainable solutions for clinical applications.\n                  <\/jats:p>","DOI":"10.1002\/jemt.24801","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T02:05:46Z","timestamp":1739412346000},"page":"1795-1805","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Phytochemical\u2010Mediated Synthesis and Characterization of Silver Nanoparticles Using\n                    <i>Mirabilis jalapa<\/i>\n                    Leaf Extract and Their Antibacterial"],"prefix":"10.1002","volume":"88","author":[{"given":"Sania","family":"Rauf","sequence":"first","affiliation":[{"name":"Department of Biotechnology Mirpur University of Science and Technology  Mirpur Azad Kashmir Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7785-4260","authenticated-orcid":false,"given":"Hajra","family":"Hameed","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Health Science Centre Shenzhen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Tariq","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Mirpur University of Science and Technology  Mirpur Azad Kashmir Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afshan","family":"Afareen","sequence":"additional","affiliation":[{"name":"Department of Biotechnology University of Kotli  Kotli Azad Kashmir Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saad","family":"Gulfaraz","sequence":"additional","affiliation":[{"name":"Department of Medicine Qiqihar Medical University  Qiqihar China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noorah A.","family":"AlKubaisi","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed S.","family":"Elshikh","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano14171425"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph17091134"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2021.111967"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmrt.2020.06.074"},{"key":"e_1_2_11_6_1","first-page":"1","volume-title":"Methods in Microbiology","author":"Ball A. 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