{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T17:02:31Z","timestamp":1791910951492,"version":"4.3.4"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Arrhythmia is a common and serious global health problem, contributing to cardiovascular morbidity and mortality. The cardiac muscle is susceptible to ischemia\u2013reperfusion (I\/R) injury, which can lead to fatal arrhythmias during open\u2010heart surgery. We investigated the potential prophylactic effect of angiotensin 1\u20137 (Ang 1\u20137) using an in\u00a0vivo rat model of I\/R injury and examined the underlying mechanisms. Rats were treated with Ang 1\u20137 (1\u2009mg\/kg, IP) 30\u2009min before the surgical procedures. Twenty\u2010four rats were equally divided into four groups: sham control, sham\u2010treated with Ang 1\u20137, I\/R injury group, and I\/R injury group treated with Ang 1\u20137. In\u00a0vivo I\/R injury was induced by clamping the left coronary artery for 30\u2009min, followed by 1\u2009hour of reperfusion. The I\/R group showed abnormal electrophysiological changes and arrhythmic episodes during electrocardiography (ECG) recording, increased oxidative stress, downregulation of peroxisome proliferator\u2010activated receptor gamma (PPAR\u2010\u03b3), and upregulation of C\u2010X\u2010C motif chemokine ligand 16 (CXCL16) expression in cardiac tissue, which increased cardiac NF\u2010kB expression and IL\u201017 levels. Moreover, I\/R injury caused significant histological disruption and increased cyclooxygenase 2 (COX\u20102) and heat shock protein 90 (HSP90) immunoreactions, correlating with the extent of cardiac damage. However, preoperative Ang 1\u20137 administration significantly improved the electrophysiological, biochemical, and histopathological changes induced by I\/R injury. This study demonstrated that Ang 1\u20137 exerted protective anti\u2010arrhythmic, anti\u2010inflammatory, and pro\u2010healing effects by upregulating PPAR\u2010\u03b3 and downregulating CXCL16, IL\u201017, and NF\u2010kB pathways, suggesting it is a promising cardioprotective agent for preventing arrhythmias induced by I\/R injury.<\/jats:p>","DOI":"10.1002\/jemt.24812","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T23:54:09Z","timestamp":1738022049000},"page":"1643-1653","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Angiotensin 1\u20137 Attenuates the Development of Ischemia\u2013Reperfusion\u2010Induced Arrhythmia in Rats: Electrophysiology, Molecular, and Immunohistochemical Study"],"prefix":"10.1002","volume":"88","author":[{"given":"Amy F.","family":"Boushra","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghada Farouk","family":"Soliman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walaa","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laila Ahmed","family":"Rashed","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman Ali Abd","family":"El\u2010Meguid","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Medicine Fayoum University  Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yara S.","family":"Abouelela","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noha A. 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