{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T01:09:03Z","timestamp":1789002543672,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T00:00:00Z","timestamp":1740182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp; Technique"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>This study provides a detailed histological and immunohistochemical analysis of the kidney and adrenal gland in <jats:styled-content style=\"fixed-case\">\n<jats:italic>Varanus niloticus<\/jats:italic>\n<\/jats:styled-content> (Nile monitor lizard), highlighting their structural features and adaptive mechanisms. Ten adult female Nile monitors were collected from Qena Province, Egypt, and their kidneys and adrenal glands were examined. Kidney tissues were processed for histological analysis, and immunohistochemistry was performed to evaluate the expression of key markers, including vimentin, E\u2010cadherin, CK7, and NSE. In contrast to other reptiles, the kidney was divided into the cortex and medulla and contained a loop of Henle. The cortex contains renal corpuscles and nephron tubules, while the medulla primarily comprises collecting ducts. The proximal tubules were lined with acidophilic cuboidal cells, whereas the distal tubules exhibited pale cuboidal cells with fewer microvilli. Immunohistochemistry revealed vimentin expression in podocytes and a few renal tubule epithelial cells, while E\u2010cadherin was expressed in the distal tubules, loop of Henle, and collecting ducts. NSE was strongly expressed in the renal corpuscles and macula densa of the juxtaglomerular apparatus, as well as in the peripolar cells, but was absent in the proximal tubules. CK7 was predominantly expressed in the distal tubules and collecting ducts. The adrenal glands comprise steroidogenic and chromaffin cells associated with the posterior cardinal veins of the kidney. NSE was strongly expressed in chromaffin cells, while vimentin was detected in steroidogenic cells. E\u2010cadherin and CK7 are not expressed in the adrenal tissues. These findings provide insights into the structural and functional adaptations of the kidney and adrenal glands in Nile monitors, offering a foundation for future research into the comparative anatomy and functional ecology of reptilian excretory and endocrine systems.<\/jats:p>","DOI":"10.1002\/jemt.24825","type":"journal-article","created":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T07:39:26Z","timestamp":1740209966000},"page":"1858-1868","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["\u201cHistological and Immunohistochemical Characterization of the Kidney and Adrenal Gland in Nile Monitor Lizards (<i>Varanus niloticus<\/i>)\u201d"],"prefix":"10.1002","volume":"88","author":[{"given":"Doaa M.","family":"Mokhtar","sequence":"first","affiliation":[{"name":"Department of Cell and Tissues, Faculty of Veterinary Medicine Assuit University  Assiut Egypt"},{"name":"Department of Histology and Anatomy, School of Veterinary Medicine Badr University in Assiut  Assiut Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed A.","family":"Abdel\u2010Ghani","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine King Faisal University  AL\u2010Ahsa Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Abdelsabour","family":"Khalaf","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine South Vally University  Qena Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid M.","family":"Alkhodair","sequence":"additional","affiliation":[{"name":"Department of Anatomy, College of Veterinary Medicine King Faisal University  Al\u2010Ahsa Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enas A.","family":"Abdelhafez","sequence":"additional","affiliation":[{"name":"Department of Cell and Tissues, Faculty of Veterinary Medicine Assuit University  Assiut Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,22]]},"reference":[{"issue":"4","key":"e_1_2_11_2_1","first-page":"599","article-title":"Comparative Study on the Kidneys of Ptychadena mascarenensis and Chalcides ocellatus ocellatus in Relation to Their Environment","volume":"58","author":"Abdel Moghith Z. 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