{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:41:44Z","timestamp":1787168504531,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,3,3]],"date-time":"2025-03-03T00:00:00Z","timestamp":1740960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microscopy Res &amp;amp; Technique"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We conducted a study to validate the automated scoring of Fluorescent in Situ Hybridization (FISH) in a routine cytogenetics laboratory using selected CD138\u2010positive cells in samples from patients with multiple myeloma. A workstation was optimized based on the manufacturer's configurations. Six commercial probes (\n                    <jats:italic>CDKN2C\/CKS1B<\/jats:italic>\n                    ,\n                    <jats:italic>RB1\/DLEU1\/LAMP1<\/jats:italic>\n                    ,\n                    <jats:italic>TP53\/CEN17<\/jats:italic>\n                    ,\n                    <jats:italic>FGFR3::IGH<\/jats:italic>\n                    ,\n                    <jats:italic>CCND1::IGH<\/jats:italic>\n                    , and\n                    <jats:italic>IGH::MAF<\/jats:italic>\n                    ) were examined to detect gains, losses, and rearrangements of genes across a total of 180 slides. We used reference values proposed by the European Myeloma Network (10% for rearrangements and 20% for gains and losses) to compare the accuracy of manual, automated, and semi\u2010automated (automated coupled with manual revision) scoring. The time spent by the biologist to perform semi\u2010automated and manual scoring was compared. Automated scoring was not effective and lacked validation. In contrast, semi\u2010automated scoring proved to be efficient and highly accurate for all probes and offered time\u2010saving benefits for deletion\/gain probes. These findings suggest that semi\u2010automated iFISH scoring for multiple myeloma is feasible and could become a routine practice in cytogenetics laboratories.\n                  <\/jats:p>","DOI":"10.1002\/jemt.24844","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T16:53:03Z","timestamp":1741711983000},"page":"2001-2008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Semi\u2010Automated Interphase\n                    <scp>FISH<\/scp>\n                    (\n                    <scp>iFISH<\/scp>\n                    ) Spot Scoring in\n                    <scp>CD138<\/scp>\n                    \u2010Positive Cells: Validation Study for Genetic Abnormalities Detection in Multiple Myeloma"],"prefix":"10.1002","volume":"88","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4543-4905","authenticated-orcid":false,"given":"Daniela","family":"Borri","sequence":"first","affiliation":[{"name":"Hospital Israelita Albert Einstein Laborat\u00f3rio Cl\u00ednico  S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Universidade de S\u00e3o Paulo, Hospital das Cl\u00ednicas Faculdade de Medicina  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin Jia","family":"Lin","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo, Hospital das Cl\u00ednicas Faculdade de Medicina  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Camillo","sequence":"additional","affiliation":[{"name":"Metasystems  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata K.","family":"Kishimoto","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein Laborat\u00f3rio Cl\u00ednico  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauren F. M.","family":"Santos","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein Laborat\u00f3rio Cl\u00ednico  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta M.","family":"Safranauskas","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein Laborat\u00f3rio Cl\u00ednico  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria G.","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein Laborat\u00f3rio Cl\u00ednico  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason de L.","family":"Silva","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein Laborat\u00f3rio Cl\u00ednico  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda A. C.","family":"Coimbra","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein Laborat\u00f3rio Cl\u00ednico  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilmara de S. E.","family":"Silva","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein Laborat\u00f3rio Cl\u00ednico  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murilo C.","family":"Cervato","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein VarsOmics  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira D. R. P.","family":"Velloso","sequence":"additional","affiliation":[{"name":"Hospital Israelita Albert Einstein Laborat\u00f3rio Cl\u00ednico  S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Universidade de S\u00e3o Paulo, Hospital das Cl\u00ednicas Faculdade de Medicina  S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20557"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102006\u201008\u2010040410"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01121"},{"key":"e_1_2_11_5_1","unstructured":"Bras\u00edlia.2024.\u201cConitec.\u201d Brazil.https:\/\/www.gov.br\/conitec\/pt\u2010br."},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2024.101168"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.21.02614"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1755\u20108166\u20102\u201012"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42649\u2010021\u201000053\u2010y"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185\u2010022\u201003353\u20105"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPF7NFLW8UAJEP"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mne.2018.10.006"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20260"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjhh.2016.01.005"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/GIM.0b013e3182227295"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2013.01.019"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22082"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2022018339"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.64.2.600"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.056176"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/geu008"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.34119\/bjhrv6n3\u201029"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.31487\/j.EJMC.2020.01.01"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.gim.0000195645.00446.61"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2007.060128"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5371"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphg.96"}],"container-title":["Microscopy Research and Technique"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jemt.24844","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T13:50:07Z","timestamp":1760622607000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jemt.24844"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,3]]},"references-count":26,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/jemt.24844"],"URL":"https:\/\/doi.org\/10.1002\/jemt.24844","archive":["Portico"],"relation":{},"ISSN":["1059-910X","1097-0029"],"issn-type":[{"value":"1059-910X","type":"print"},{"value":"1097-0029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,3]]},"assertion":[{"value":"2024-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}